摘要
本文以多肉植物筒叶花月为材料,利用植物离体组织培养技术,对筒叶花月离体叶片进行了初代培养物的诱导。结果表明:叶片采用竖放方式,诱导培养基为:MS+KT 1 mg/L+6-BA 1.2 mg/L+NAA 2 mg/L,适合筒叶花月叶片初代培养物愈伤组织的诱导;叶片采用平放方式,诱导培养基为:MS+KT 1 mg/L+6-BA 1.2 mg/L+NAA 0.5 mg/L,适合筒叶花月初代培养物不定芽的诱导。
This paper took the Crassula oblique Gollum as the object and studied the induction of the initial culture of the in vitro leaves by the in vitro tissue culture technology. The results showed that the induction medium was MS+KT 1 mg/L+6-BA 1.2 mg/L+NAA 2 mg/L when the leaves were arranged vertically, which was suitable for the callus induction of the initial culture of the leaves of the Crassula oblique Gollum. When the leaves were arranged flat, the induction medium was MS+KT 1 mg/L+6-BA 1.2 mg/L+NAA 0.5 mg/L, which was suitable for the adventitious bud induction.
作者
刘艳妮
高露
LIU Yan-ni(College of Agriculture and Bioscience Engineering, Yinchuan University of Energy, Yongning, Ningxia 750105)
出处
《宁夏农林科技》
2018年第12期7-10,共4页
Journal of Ningxia Agriculture and Forestry Science and Technology
基金
银川能源学院校级科研项目"几种新兴多肉植物快繁技术研究"(2016-KY-Y-14)
关键词
筒叶花月
初代培养物
愈伤组织
不定芽
Crassula oblique Gollum
Initial culture
Callus tissue
Adventitious bud
