口服液类保健食品中18种重金属元素检测

Study on 18 Heavy Metal Elements in Oral Liquid Health Foods

本文旨在建立一种基于微波消解-电感耦合等离子质谱(Inductively coupled plasma mass spectrometry,ICP-MS)测定源于中草药的口服液类保健食品中18种重金属元素(Pb、Cd、As、Hg、Cu、Pt、Pd、Ir、Rh、Ru、Os、Mo、Ni、Cr、V、Mn、Fe、Zn)的方法。采用微波消解法处理口服液样品,以Ge、In、Bi元素作为校正基体效应与信号漂移的内标元素,使用ICP-MS对十批样品中18种重金属进行含量测定。结果显示18种重金属标准曲线线性关系良好,R2≥0.999,各元素的检出限在0.0001～2.743 ng/mL之间,精密度RSD在0.49%～4.29%之间,重复性RSD值在1.2%～15%之间,加样回收率在82.74%～122.7%之间;检测两种口服液中18种重金属元素均未超标。表明该方法操作简便、分析速度快、灵敏度高、准确性好,适用于口服液类保健食品中重金属检测,可为保健食品安全性评价提供依据。

A kind of heavy metal elements(Pb,Cd,As,Hg,Cu,Pt,Pd,Ir,Rh,Ru,Os,Mo,Ni,Cr,V,Mn,Fe,Zn)in oral liquid health foods derived from Chinese herbal medicine based on inductively coupled plasma mass spectrometry(ICP-MS)was established in this work.The oral liquid samples were pre-treated by microwave digestion.The Ge,In and Bi elements were used as the internal standard elements for correcting the matrix effect and signal drift.The contents of 18 heavy metals in ten batches of samples were determined by ICP-MS.Results showed that good linear relations of 18 heavy metal standard curves were found,R^2≥0.999,the detection limit of each element was between 0.0001~2.743ng/mL,the precision RSD was between 0.49%~4.29%,and the repetitive RSD value was between 1.2%and 15%,the recovery rate of the sample was between 82.74%and 122.7%.The 18 heavy metal elements in the two oral liquids were not exceeded.Results showed that this method is easy to operate,fast in analysis,high in sensitivity and good in accuracy.It is suitable for the detection of heavy metals in oral liquid health foods,and can provide a basis for health food safety evaluation.