藤茶总黄酮提取物对糖尿病前期小鼠的干预作用

Preventive and Therapeutic Effect of Total Flavonoid Extract from Vine Tea on Mice with Prediabetes

本实验探究通过饮用藤茶的生活干预方式预防、延缓或治愈糖尿病前期的可行性。本实验以高脂饮食结合注射链脲佐菌素的方式构建了小鼠糖尿病前期模型,成模后将小鼠分为空白组、模型组、藤茶总黄酮提取物低剂量组(200 mg/kg mb)和高剂量组(500 mg/kg mb)、阿卡波糖组和阿卡波糖-藤茶总黄酮提取物联用组进行干预,并检测了小鼠的相关指标。研究结果表明:与模型组相比,高剂量干预组小鼠的空腹血糖、葡萄糖耐受性、糖化血清蛋白及胰岛素分泌水平均显著降低(P<0.05);干预组小鼠的炎症因子(白细胞介素4、肿瘤坏死因子、C反应蛋白)、血浆脂质指标(游离脂肪酸、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇)、短链脂肪酸(乙酸、丙酸、丁酸)水平与模型组间均存在统计学差异(P<0.05)。一定剂量的藤茶黄酮提取物对糖尿病前期小鼠显示出有效干预作用,其可能通过抗炎、调节糖脂代谢及肠道菌群等方式来延缓或阻止小鼠糖尿病的进一步发展。

In order to explore the feasibility of preventing,delaying or curing prediabetes by drinking vine tea,a mouse model of prediabetes was established by feeding a high-sugar and high-fat diet and injection of streptozocin(STZ).Then the model mice were divided into model group,low-dose(200 mg/kg mb)and high-dose(500 mg/kg mb)groups orally administered with total flavonoid extract from vine tea,acarbose control group and acarbotane-flavonoid extract group.Parameters of interest were detected.The results showed that fasting blood glucose,glucose tolerance,glycosylated serum protein(GSP)and insulin secretion levels in the high-dose treatment group were significantly lower than those in the model group(P<0.05).In addition,there was a statistical difference in inflammatory factors(interleukin 4,tumor necrosis factor-αand C reactive protein),plasma lipid indices(free fatty acids,triglyceride,low density lipoprotein cholesterol,total cholesterol and high density lipoprotein cholesterol)and cecal short chain fatty acid levels(acetic acid,propionic acid,and butyric acid)between the model and treatment groups(P<0.05).A certain dose of total flavonoid extract from vine tea could delay or prevent the development of diabetes in mice by exerting anti-inflammatory activity and regulating glycolipid metabolism and the intestinal microflora.