甘薯汁对果蝇寿命及抗氧化能力的影响

Effect of Sweet Potato Juice on Life Span and Antioxidant Capacity in Drosophila melanogaster

为探讨甘薯汁抗氧化及防衰老的功效,选取果蝇为动物模型,在培养基中分别添加低、中、高剂量(分别为0.10、0.15、0.20 mg/mL)甘薯汁饲喂8 h内羽化未交配的雌、雄果蝇,通过果蝇生存实验及测定不同饲养时间果蝇体内的超氧化物歧化酶(superoxide dismutase,SOD)活力、总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malondialdehyde,MDA)含量,评价甘薯汁对果蝇寿命和抗氧化酶活性的影响。结果表明:与对照组相比,不同剂量甘薯汁组均能显著延长雌、雄果蝇的平均寿命(P<0.05或P<0.01),且呈剂量依赖性。果蝇SOD活力、T-AOC在低、中、高剂量组中均显著增加(P<0.05或P<0.01),中、高剂量组果蝇体内MDA含量显著降低(P<0.05或P<0.01)。其中,与对照组相比,高剂量组可使雌、雄果蝇平均寿命分别延长17.93%和16.00%,SOD活力分别提高50.51%、58.61%,T-AOC分别提高79.73%、62.67%,MDA含量分别下降35.59%、37.14%。结论:甘薯汁可以提高果蝇体内的抗氧化能力,具有一定的延缓衰老作用。

In order to investigate the antioxidant and anti-aging effect of sweet potato juice,Drosophila melanogaster was chosen as an animal model.Male and female Drosophila melanogaster with eclosion and without mating within 8 h were fed on a diet supplemented with low,moderate or high doses(0.10,0.15,and 0.20 mg/mL)of sweet potato juice.The survival rate of D.melanogaster was investigated,and the activity of superoxide dismutase(SOD),total antioxidant capacity(T-AOC)and malondialdehyde(MDA)content were measured in its homogenate.The results showed that compared with the control group,average life span and maximum life span in all dose groups were significantly increased(P<0.05 or P<0.01)in a dose-dependent manner.SOD activity and T-AOC were significantly increased in all dose groups(P<0.05 or P<0.01),whereas MDA content was significantly decreased in the middle-and high-dose groups(P<0.05 or P<0.01).Compared with the control group,average life span in the high-dose group was extended by 17.93%and 16.00%for female and male flies,the activity of SOD was increased by 50.51%and 58.61%,T-AOC activity was increased by 79.73%and 62.67%,while the content of MDA was decreased by 35.59%and 37.14%,respectively.In conclusion,sweet potato juice can prolong life span and delay aging in D.melanegaster.