环状RNA ciRS-7在三阴性乳腺癌中的表达及其对细胞侵袭和迁移的影响

Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative Breast Cancer Cells

目的探讨环状RNA ciRS-7在三阴性乳腺癌(TNBC)中的表达及其对细胞侵袭和迁移的影响。方法通过qRT-PCR法检测乳腺癌组织和细胞中ciRS-7的表达水平,分析其与乳腺癌患者临床病理指标之间的关系。用划痕实验和Transwell实验检测沉默ciRS-7后MDA-MB-231和BT-549细胞的迁移和侵袭能力的变化。利用动物实验在体内进行验证。结果 TNBC组织中ciRS-7的相对表达量为(6.52±0.38),显著高于Luminal型(1.56±0.17)(P<0.001)和HER2过表达型乳腺癌组织(2.27±0.66)(P<0.001)以及癌旁正常组织(0.83±0.09)(P<0.001)。ciRS-7表达与分子分型、肿瘤浸润和淋巴结转移明显相关(均P<0.05);而与患者年龄、肿瘤直径和病理分级无关(均P>0.05)。沉默ciRS-7后,MDA-MB-231和BT-549细胞的迁移和侵袭明显减弱(均P<0.05)。ciRS-7敲低组肺转移瘤数明显减少(P<0.05)。结论 ciRS-7在TNBC中高表达,并可能增强TNBC细胞的侵袭和迁移。

Objective To investigate the expression of circular RNA ciRS-7 in triple-negative breast cancer(TNBC)and its effect on invasion and migration.Methods The expression levels of ciRS-7 in TNBC tissues and cells were detected by qRT-PCR,and its relationship with clinicopathological parameters of breast cancer patients was further analyzed.Scratch and Transwell assays were used to detect the migration and invasion of MDA-MB-231 and BT-549 cells after silencing ciRS-7.Animal experiment was performed to verify the above findings in vivo.Results The relative expression of ciRS-7 in TNBC tissues was(6.52±0.38),significantly higher than Luminal type(1.56±0.17)(P<0.001),HER2 overexpressing breast cancer tissues(2.27±0.66)(P<0.001)and normal adjacent tissues(0.83±0.09)(P<0.001).Clinical data analysis showed that ciRS-7 expression was significantly associated with molecular typing,tumor invasion and lymph node metastasis(all P<0.05),not associated with patients'age,tumor diameter or pathological grade(all P>0.05).After silencing ciRS-7,the migration and invasion of MDA-MB-231 and BT-549 cells were significantly reduced(all P<0.05).The number of lung metastasis clones was significantly reduced in ciRS-7 knockdown group(P<0.05).Conclusion ciRS-7 is highly expressed in TNBC and may enhance the invasion and migration of TNBC cells.