人参皂甙Rh2对人胶质瘤细胞U87MG凋亡的影响

Effects of genoside Rh2 on the apoptosis of human brain glioma cells U87MG

目的观察人参皂甙Rh2对胶质瘤细胞凋亡的影响并初步探讨其可能机制。方法将培养的人胶质瘤细胞U87MG随机分为人参皂甙Rh2组、人参皂甙Rh2+尼莫地平组和对照组。人参皂甙Rh2组在常规培养细胞时加入20μg/ml的人参皂甙Rh2,人参皂甙Rh2+尼莫地平组在人参皂甙Rh2组培养细胞时加入浓度为10μmol/L的尼莫地平。利用流式细胞仪检测U87MG细胞凋亡,利用激光共聚焦显微镜和流式细胞仪检测U87MG细胞内钙离子浓度。结果与对照组相比,人参皂甙Rh2促进U87MG细胞凋亡(P<0.05),且增加细胞内游离钙离子浓度(P<0.05);尼莫地平显著减少人参皂甙Rh2引起的U87MG细胞凋亡(P<0.05)。结论人参皂甙Rh2可以通过增加细胞内游离钙离子浓度促进U87MG细胞凋亡。

Objective To investigate the effects of genoside Rh2 on the apoptosis of human brain glioma cells and its mechanism. Methods Human brain glioma cells U87MG were randomly divided into two groups, i.e. experimental group in which U87MG cells were cultured in the medium containing 20 μg/ml genoside Rh2 and control group in which U87MG cells were cultured in the medium containing isodose physiological salt solution. The apoptosis of U87MG cells was determined by flow cytometry. And then the intracellular calcium concentration of U387MG cells was measured by laser scanning confocal microscope and flow cytometry. Results The rate of U87MG cells apoptosis and intracellular calcium concentration were significantly higher in the experimental group than those in the control group (P<0.05). Moreover the intracellular calcium concentration and the apoptosis rate of U87MG cells raised by genoside Rh2 were significantly decreased by nifedipine [(a specitic inhibitor of Ca++ channel (P<0.05)]. Conclusion Genoside Rh2 can promote apoptosis of human brain glioma U87MG cells by the up-regulation of intracellular calcium concentration.