基于iTRAQ技术的大鼠挤压伤血清蛋白质组学分析

Serum Proteomics Analysis of Rats with Crush Injury Based on iTRAQ Technology

目的运用蛋白质组学技术对比筛选挤压伤大鼠模型的血清差异蛋白。方法选取20只Wistar大鼠随机分为挤压伤组和对照组,每组10只。采用同位素标记相对和绝对定量技术以及液相色谱-串联质谱技术对2组的血清进行差异蛋白分析。使用Thermo Xcalibur 4.0软件采集数据,选用UniProt-Rattus norvegicus数据库进行搜索,并对差异表达的蛋白进行基因本体论富集分析以及差异蛋白通路富集分析。结果 2组血清中共有差异表达的蛋白206个,其中133个表达上调主要有14-3-3蛋白家族、M型肌酸激酶等,73个表达下调主要有小鼠球蛋白-1、血清白蛋白等。差异蛋白上调的通路共144种,下调的通路有89种。结论筛选出的差异表达蛋白可能与挤压伤的发展及预后有关,可能成为临床早期诊断及治疗的分子生物学标志物。

Objective To compare and screen serum differential proteins in rat models with crush injury by using proteomics technology. Methods A total of 20 Wistar rats were randomly divided into crush injury group and control group ( n =10 rats for each group). Isotope labeling relative and absolute quantitative techniques and liquid chromatography-tandem mass spectrometry were used to analyze differential proteins in serum in two groups. Thermo Xcalibur 4.0 software was used to collect data, and UniProt-Rattus norvegicus database was used to search, and then gene ontology enrichment analysis and differential protein pathway enrichment analysis were performed for differentially expressed proteins. Results There were 206 differentially expressed proteins in two groups, among which 133 up-regulated proteins were mainly 14-3-3 protein family, M-type creatine kinase and so on, and 73 down-regulated proteins were mainly mouse globulin-1, serum albumin and so on. There are 144 up-regulated pathways and 89 down-regulated pathways for differential proteins. Conclusion Differentially expressed proteins by screening may be related to development and prognosis of crush injury, and they may be molecular biomarkers for early diagnosis and treatment.