不同产地野生黄姜中薯蓣皂苷含量的比较

Comparison of Diosgenin Content in Wild Dioscorea zingiberensis from Different Habitats

目的:建立黄姜中薯蓣皂苷含量测定的方法,并对不同产地野生黄姜中薯蓣皂苷含量进行比较。方法:采用高效液相色谱(HPLC)法,色谱柱为Inertsil ODS-3,流动相为乙腈-水(50∶50,V/V),流速为1.0 mL/min,柱温为30℃,检测波长为203 nm,进样量为10μL。采用建立的方法测定不同产地(陕西商洛、陕西安康、河南内乡、云南宣威、四川德阳、湖北十堰、湖南张家界、湖南常德)野生黄姜中薯蓣皂苷的含量,并比较其差异。结果:薯蓣皂苷检测质量浓度线性范围为4.16～165.6μg/mL(r=0.999 9),精密度、重复性、稳定性试验的RSD均<2%(n=5或n=6),薯蓣皂苷平均回收率为101.18%(RSD=1.27%,n=6)。不同产地野生黄姜中薯蓣皂苷含量由高到低依次为四川德阳(1 405.36μg/g)>陕西商洛(1 201.79μg/g)>湖南张家界(1 035.18μg/g)>陕西安康(632.64μg/g)>湖南常德(598.64μg/g)>云南宣威(425.34μg/g)>河南内乡(350.13μg/g)>湖北十堰(338.39μg/g)。结论:该法操作简便、准确,可用于黄姜中薯蓣皂苷含量的测定;不同产地野生黄姜中薯蓣皂苷含量差异明显,其中四川德阳产地的薯蓣皂苷含量最高。

OBJECTIVE:To establish a method for the content determination of diosgenin in Dioscorea zingiberensis,and to compare the contents of diosgenin in wild D.zingiberensis from different habitats.METHODS:HPLC method was adopted.The determination was performed on Inertsil ODS-3 column with mobile phase consisted of acetonitrile-water(50∶50,V/V)at the flow rate of 1.0 mL/min.The column temperature was set at 30℃.The detection wavelength was set at 203 nm,and sample size was 10μL.Established method was used to determine the contents of diosgenin in wild D.zingiberensis from different habitats(Shaanxi Shangluo,Shaanxi Ankang,Henan Neixiang,Yunnan Xuanwei,Sichuan Deyang,Hubei Shiyan,Hunan Zhangjiajie,Hunan Changde).The differences of diosgenin content were compared.RESULTS:The linear range of diosgenin were 4.16-165.6μg/mL(r=0.999 9).RSDs of precision,reproducible and stability tests were all lower than 2%(n=5 or n=6).The average recovery of diosgenin was 101.18%(RSD=1.27%,n=6).The content of diosgenin in wild D.zingiberensis from different habitats were in descending order,i.e.Sichuan Deyang(1 405.36μg/g)>Shaanxi Shangluo(1 201.79μg/g)>Hunan Zhangjiajie(1 035.18μg/g)>Shaanxi Ankang(632.64μg/g)>Hunan Changde(598.64μg/g)>Yunnan Xuanwei(425.34μg/g)>Henan Neixiang(350.13μg/g)>Hubei Shiyan(338.39μg/g).CONCLUSIONS:Established method is simple,accurate and suitable for the content determination of diosgenin in D.zingiberensis.The contents of diosgenin in wild D.zingiberensis from different habitats are different significantly,and the content of diosgenin in the samples from Sichuan Deyang is the highest.