摘要
目的寻找成纤维细胞与脐带间充质干细胞(UCMSC)在细胞表面蛋白与分化能力方面的差异。方法流式细胞术分析细胞表达CD105、CD90、CD73、CD44、CD14、CD34、CD45、CD79a、HLA-DR、FSP-1及TM4SF1的表达情况;细胞分化实验:观察成纤维细胞与UCMSC的成脂、成软骨及成骨能力;RT-PCR检测二者FSP-1、TM4SF1表达情况。两种细胞表型比较采用独立样本t检验。结果成纤维细胞与UCMSC的表面分子CD105、CD90、CD73、CD44、CD14、CD34、CD45、CD79a、HLA-DR表达相似(P均> 0.05),都具有成脂、成软骨、成骨的分化能力;成纤维细胞与UCMSC FSP-1阳性细胞比例分别为(98.6±0.3323)%及(98.90±0.2665)%(t=0.4677,P=0.5294); UCMSC TM4SF1阳性细胞比例为(97.23±0.2250)%,成纤维细胞TM4SF1阳性细胞比例为(0.0082±0.0018)%(t=346.9,P <0.01)。结论 TM4SF1在成纤维细胞与UCMSC上的表达量存在差异。
Objective To find the difference between fibroblasts and umbilical cord mesemchemal stem cells(UCMSC)in surface markers and differentiation.Methods Flow cytometric analysis of CD105,CD90,CD73,CD44,CD14,CD34,CD45,CD79a,HLA-DR,FSP-1 and TM4SF1 was performed for fibroblasts and UCMSC.Multilineage differentiation of fibroblasts and UCMSC into fat cells,cartilage cells and bone cells were induced.The expression of FSP-1 and TM4SF1 in fibroblasts and in UCMSC was assayed by RT-PCR.Data analysis between different groups was perfermed with independent-sample t test.Results Those markers which were found to be expressed on UCMSC were also expressed on fibroblasts in comparable levels(P>0.05).Fibroblasts and UCMSC could differentiate into fat cells,cartilage cells and bone cells in vitro.Both fibroblasts and UCMSC were positive for FSP-1(98.6±0.3323)﹪vs(98.90±0.2665)﹪,(t=0.4677,P=0.5294).TM4SF1 was positive on UCMSC(97.23±0.2250)﹪,but negative on fibroblast(0.0082±0.0018)﹪(t=346.9,P<0.01).Conclusion There is a significant difference in expression of TM4SF1 between fibroblasts and UCMSC.
作者
杨娟
柴勋
刘根桃
Yang Juan;Chai Xun;Liu Gentao(Shanghai Biomed-union Biote Chnology Co.,Ltd,Shanghai 201321,China)
出处
《中华细胞与干细胞杂志(电子版)》
2018年第5期278-283,共6页
Chinese Journal of Cell and Stem Cell(Electronic Edition)