结肠癌组织血管生成抑制素结合蛋白、迁移诱导蛋白-7、MMP-2表达及其与血管生成拟态的相关性

Expression of AMOT,MIG-7,and MMP-2 in colon cancer and its correlation with vasculogenic mimicry

目的观察结肠癌组织血管生成抑制素结合蛋白(AMOT)、迁移诱导蛋白-7(MIG-7)、基质金属蛋白酶-2(MMP-2)的表达变化,并分析其与血管生成拟态(VM)的相关性。方法 70例结肠癌患者,均行肿瘤切除术,术中保留癌组织及癌旁正常组织。采用免疫组化法检测癌组织与癌旁组织AMOT、MIG-7及MMP-2。采用双重染色法检测癌组织VM,并计算癌组织VM阳性率。采用Spearman法分析AMOT、MIG-7及MMP-2与结肠癌患者VM的相关性,Kaplan Meier及Cox回归模型分析AMOT、MIG-7、MMP-2、VM及结肠癌临床病理参数与预后的关系。结果结肠癌癌组织AMOT、MIG-7、MMP-2阳性率分别为58. 6%(41/70)、61. 4%(43/70)、70. 0%(49/70),癌旁组织均分别为31. 4%(22/70)、0、4. 3%(3/70)。与癌旁组织比较,癌组织AMOT、MIG-7、MMP-2阳性率均较高(P均<0. 05)。结肠癌组织VM阳性率32. 9%(23/70)。AMOT表达与结肠癌患者分化程度及淋巴结转移均有关(P均<0. 05); MIG-7、MMP-2表达及VM量与结肠癌患者分化程度、浸润深度及淋巴结转移均有关(P均<0. 05)。结肠癌组织MIG-7、MMP-2表达与VM均呈正相关(r分别为0. 304,0. 259; P均<0. 05)。分化程度、浸润深度、淋巴结转移、MIG-7表达水平与结肠癌患者的预后有关。年龄、分化程度、淋巴结转移、MIG-7蛋白水平及VM阳性是结肠癌预后的独立危险因素。结论结肠癌组织AMOT、MIG-7、MMP-2均呈高表达,VM阳性表达较高。MIG-7、MMP-2可能通过调控结肠癌VM的形成,影响结肠癌的侵袭、转移及预后。

ObjectiveTo observe the expression of angiomotin(AMOT),migration inducting gene-7(MIG-7),and matrix metalloproteinase 2(MMP-2)in the colon cancer tissues and to analyze their correlation with vasculogenic mimicry(VM).Methods Seventy patients with colorectal cancer underwent tumor resection,and cancer tissues and adjacent normal tissues were preserved during operation.Immunohistochemistry was used to detect the expression of AMOT,MIG-7 and MMP-2 in the cancer and adjacent tissues,and VM was detected by double staining to calculate the positive rate of VM.In addition,we analyzed the relationships of AMMOT,MIG-7,and MMP-2 with VM by Spearman.Kaplan Meier and Cox regression models were used to analyze the relationships between the clinicopathological parameters,AMOT,MIG-7,MMP-2,VM and the prognosis of colon cancer.Results The positive rates of AMOT,MIG-7,and MMP-2 were 58.6%(41/70),61.4%(43/70),and 70.0%(49/70),in the cancer tissues,versus 31.4%(22/70),0,and 4.3%(3/70)in the adjacent tissues,respectively.Compared with the adjacent control tissues,the positive rates of AMOT,MIG-7,and MMP-2 in the cancer tissues were higher(all P<0.05).The positive rate of VM in the colon cancer tissues was 32.9%(23/70).The expression of AMOT was correlated with the degree of differentiation and lymph node metastasis(both P<0.05),and the expression of MIG-7,MMP-2,and the amount of VM were correlated with the degree of differentiation,depth of invasion,and lymph node metastasis(all P<0.05).There was a positive correlation between MIG-7,MMP-2 and VM in the colon cancer tissues(r=0.304 and 0.259,respectively;all P<0.05).The degree of differentiation,depth of invasion,lymph node metastasis,and MIG-7 expression were related to the prognosis of colon cancer patients.Age,differentiation,lymph node metastasis,the expression of MIG-7,and VM were independent risk factors for prognosis of colon cancer.ConclusionsAMOT,MIG-7 and MMP-2 are highly expressed in the colon cancer tissues,and the positive expression of VM is higher;MIG-7 and MMP-2 may affect the invasion,metastasis and prognosis of colon cancer by regulating the formation of VM in colon cancer.