p44/WDR77在非小细胞肺癌中表达及其与预后的关系

Expression of p44/WDR77 in non-small cell lung cancer and its relationship with prognosis

目的本研究旨在探讨非小细胞肺癌(non-small cell lung cancer, NSCLC)中p44/WDR77表达状况及其与临床病理分化、肿瘤TNM分期及患者预后之间的关系。方法采用免疫组化EnVinsion法检测p44/WDR77在192例患者NSCLC组织和癌旁正常组织中的表达情况,分析其表达与NSCLC病理分化、肿瘤TNM分期及患者预后之间的关系。结果在192例NSCLC患者肿瘤组织中,p44/WDR77的阳性表达率为81.3%(156/192),在与之对应的癌旁正常组织中的阳性表达率为5.2%(10/192),二者比较差异显著(P<0.001)。p44/WDR77在肺癌组织中高表达主要定位于胞核(鳞癌)和胞质(腺癌)中。鳞癌和腺癌组织中p44/WDR77的阳性表达率比较差异无统计学(P=0.067);p44/WDR77在高、中、低三种不同病理分化的NSCLC组织中阳性表达率分别为58.3%(7/12)、79.9%(115/144)、94.4%(34/36),差异有统计学意义(P=0.003);在临床肿瘤TNM不同分期的NSCLC组织中,p44/WDR77的阳性表达率分别为Ⅰ期77.1%(27/35)、Ⅱ期73.1%(49/67)、Ⅲ期85.7%(60/70)、Ⅳ期100%(20/20),表达差异显著(P<0.001);p44/WDR77的表达差异与患者的性别,解剖学肿瘤分类(中心型、周围型)均无统计学意义(P=0.861、P=0.462),而与NSCLC患者年龄、淋巴结转移与否、病灶范围显著相关(P<0.001、P=0.036、P=0.041)。在随访的192例NSCLC病例中,p44/WDR77表达阳性和阴性的中位生存期(median survival time, MST)分别为21.3和37.5个月,差异具有统计学意义(P=0.049)。结论检测p44/WDR77的表达水平可能在NSCLC的诊断、恶性程度的预判、病灶转移及生存预后方面的预测具有重要的意义,有望成为诊断NSCLC和患者预后的潜在肿瘤标志物。

Objective To evaluate the expression of p44/WDR77 in non-small cell lung cancer(NSCLC)patients,and to determine its relationship with clinicopathological differentiation,TNM staging of tumor and prognosis.Methods The expression of p44/WDR77 was detected by immunohistoehemieal EnVision method in 192 pairs of NSCLC tissues and adjacent normal tissues,in order to analyze its expression and NSCLC pathological differentiation,TNM staging of the tumor and the relationship between the prognosis of patients.Results The expression rate of P44/wdr77 gene was 81.3%(n=156)in 192 NSCLC samples,which showed extremely significance compared with the corresponding normal lung tissue(P<0.001).The high expression of p44/WDR77 in lung cancer tissues was mainly localized in cytoplasm(squamous cell carcinoma)and cytoplasm(adenocarcinoma).There was no statistical difference in p44/WDR77 positive expression between the squamous carcinoma group and the adenocarcinoma group(P=0.067).The positive expression rate of p44/WDR77 in high,medium and low NSCLC tissues were 58.3%(7/12),79.9%(115/144),and 94.4%(34/36),respectively.The positive expression rate of p44/WDR77 was 77.1%(27/35)at stage Ⅰ,73.1%(49/67)at stage Ⅱ,and 85.7%(60/70)at stage Ⅲ,and 100%(20/20)at stage Ⅳ(P<0.001).There was no significant difference in P44/WDR77 expression with patient's gender and tumor anatomy classification(center type,peripheral type)(P=0.861,P=0.462),but with patient's age,lymph node metastasis status and tumor size(P<0.001,P=0.036,P=0.041).Of 192 patients with follow-up,the median survival time(MST)of p44/WDR77 positive expression was better than that of negative expression(21.3 months VS 37.5 months,P=0.049).Conclusion The detection of p44/WDR77 expression may have positive significance for the diagnosis of NSCLC,malignancy,invasion and metastasis and survival prognosis,and it is expected to be an important marker for the diagnosis and prognosis of NSCLC.