槟榔中槟榔碱含量的不确定度评定

The Evaluation of Uncertainty of Arecoline Content in Areca catechu L

采用高效液相色谱法(High performance liquid chromatography,HPLC)测定槟榔中槟榔碱的含量,并对测定结果进行不确定度分析。依据JJF 1059.1—2012测量不确定度评定与表示中的方法,根据试验过程建立了数学模型,从标准储备液、标准曲线的拟合、样品的定容、样品称量、样品测量重复性及提取过程对样品回收率的影响等方面分析了引入不确定度的来源。比较得出标准曲线的拟合和样品测量重复性对合成不确定度贡献最大,达到57.7%。当槟榔中槟榔碱含量为3.31 g/kg时,其扩展不确定度为3.31±0.052 g/kg,k=2。结果表明,该评定方式适用于槟榔中槟榔碱含量的不确定度评估。

To establish a method for the determination of the arecoline in Areca catechu L.by high performance liquid chromatography and to analyze the uncertainty of the results.According to the method of Guidance on evaluating the uncertainty in Chemical Analysis,the mathematical models were established based on the experimental process,the sources of measurement uncertainty was analyzed,such as standard stock solution,fitting of standard curve,constant volume of sample,sample weighting,influence of repeatability of sample measurement and extraction process on sample recovery rate and so on.The comparison showed that the fitting of standard curve and the repeatability of sample measurement have the greatest contribution to the uncertainty of synthesis,reached 57.7%.When the content of arecoline in the sample was 3.31 g/kg k=2,its extended uncertainty introduced by the method was 0.052 g/kg.The experimental results showed that the evaluation method was applicable to the uncertainty evaluation of arecoline content in Areca catechu L.