菊苣根、茎的乙醇提取物对金黄色葡萄球菌及粪肠球菌抑菌差异性研究

Study on differences in antibacterial activity of ethanol extracts from chicory roots and stems against Staphylococcus aureus and Enterococcus faecalis

目的研究新疆出产的菊苣根、茎95%乙醇提取物对金黄色葡萄球菌和粪肠球菌抑菌效果,为明确菊苣较好的抑菌药效部位提供参考。方法牛津杯法测定根、茎提取物的抑菌圈直径;倍半稀释法测定根、茎提取物对供试菌的最小抑菌浓度(MIC);生长曲线法测定根、茎提取物对供试菌增殖的影响;酶标法测定细菌胞外溶液中碱性磷酸酶(AKP)浓度变化。结果菊苣根、茎95%乙醇提取物对金黄色葡萄球菌和粪肠球菌均有明显抑制增殖的作用;菊苣根对两种菌最小抑菌浓度均为64 g·L^(-1),菊苣茎对两种菌最小抑菌浓度分别为50 g·L^(-1)、64 g·L^(-1);菊苣茎95%乙醇提取物对金黄色葡萄球菌抑制作用较好,而菊苣根95%乙醇提取物则对粪肠球菌抑制作用更好;菊苣根乙酸乙酯萃取物对上述两种菌的抑菌效果皆优于95%乙醇提取物(P<0.01)。结论菊苣根、茎95%乙醇提取物均可明显抑制两种菌的增殖,且菊苣茎对金黄色葡萄球菌抑制作用更明显,菊苣根对粪肠球菌抑制效果较好;菊苣根最佳抑菌药效为乙酸乙酯萃取部分。

Aim To analyze the antibacterial effects of 95% ethanol extract of chicory roots and stems from Xinjiang on Staphylococcus aureus and Enterococcus faecalis ,and provide reference data for identifying the better antibacterial efficacy parts of chicory.Methods The diameters of the inhibition zone of roots and stem extracts were determined by the Oxford Cup method;the minimum inhibitory concentrations (MIC) of roots and stem extracts on the bacteria were determined by the half-dilution method;the roots and stem extracts to observe the effects on bacterial proliferation were determined by the growth curve method;and the concentration of alkaline phosphatase (AKP) in bacterial extracellular solution enzyme was determined by labeling method.Results The 95% ethanol extract of chicory roots and stems had significant inhibitory effects on the growth of Staphylococcus aureus and Enterococcus faecalis .Chicory roots’ minimum inhibitory concentration to both bacteria was 64 g·L^-1 ,and the minimum inhibitory concentration of chicory stems to both bacteria was 50 g·L^-1 ,64 g·L^-1 ,respectively.And the 95% ethanol extract of chicory stems had better inhibitory effect on Staphylococcus aureus ,while the 95% ethanol extract of chicory roots had better inhibitory effect on Enterococcus faecalis;the ethyl acetate extract of chicory roots had better antibacterial effect on both bacteria than extract of 95% ethanol did ( P <0.01).Conclusions The 95% ethanol extract of chicory roots and stems can significantly inhibit the proliferation of both bacteria,and the inhibitory effect of chicory stems on Staphylococcus aureus is more obvious,and the inhibitory effect of chicory roots on Enterococcus faecalis is better;the best antibacterial effect of chicory roots is ethyl acetate extraction.