人参皂苷Rg3对糖尿病大鼠难愈创面表皮细胞及血管新生的影响

Effects of ginsenoside Rg3 on epidermal cells and angiogenesis in impaired diabetic wound rats

目的从改善皮肤易损状态方面,探讨人参皂苷Rg3(ginsenoside Rg3, Rg3)修复糖尿病难愈创面的作用机制。方法♂SD大鼠制备糖尿病模型,随机分为模型组、氨基胍(aminoguanidirig, AG)组、Rg3高、低剂量组,另设正常组。灌胃4周后,背部皮肤等面积全层切除,伤后d 21创面皮肤取材,观测病理形态及表皮、真皮层厚度;分析表皮细胞增殖周期;检测CD31表达。结果 Rg3给药组成纤维细胞较多,坏死组织脱落及上皮匍行提前,表皮及真皮层增厚(P<0.01);表皮细胞增殖周期,模型组G_0/G_1期比例升高(P<0.01),G_2/M、S期比例降低(P<0.01),AG、Rg3组G_0/G_1期比例下降,且高剂量下降明显(P<0.05),G_2/M、S期比例升高(P<0.05或P<0.01);模型组CD31蛋白表达明显降低(P<0.01),AG、Rg3高剂组表达升高(P<0.05)。结论 Rg3通过改善皮肤病理状态,调节表皮细胞增殖周期和促进血管生成,有效修复糖尿病性皮肤损伤。

Aim To explore the repair mechanism of ginsenoside Rg3 in treating the impaired diabetic wound from improving the vulnerability of diabetic skin of rats.Methods Male SD rats( n =32) were injected intraperitoneally streptozotocin to induce hyperglycemia,except for the rats of control group( n =8).Diabetic rats were randomly divided into model group (equal volume of saline),aminoguanidine group (10 mg·kg^-1 ),ginsenoside Rg3 high dose (15 mg·kg^-1 ) and low group (5 mg·kg^-1 ).After four weeks of oral gavage treatment,full-thickness wound was established.On 21st day after injury,wound samples were collected to observe the pathological changes in wound;the thickness of epidermis and dermis was measured by HE staining;and the epidermal cell proliferation cycle was analysed by flow cytometry;the expression of CD31 in wound tissues was detected by immunohistochemical and image analytical methods.Results Ginsenoside Rg3 groups showed significantly more fibroblasts,necrotic tissue drops and advanced epithelial,and thickening of epidermis and dermis ( P <0.01).Model group showed significant increase in G 0/G 1 phase ratio of epidermal cell cycle ( P <0.01),while reduction in G 2/M and S period ratios ( P <0.01).However,G 0/G 1 period ratio decreased,while G 2/M and S period ratios rose in aminoguanidine group,ginsenoside Rg3 high dose and low dose groups.The decrease of G 0/G 1 period ratio ( P <0.05) and increase in G 2/M ( P <0.05) and S period ratios ( P <0.01) was found to be significant.The expressions of CD31 in model group was lower than those in control group ( P <0.01).Whereas,it was higher in ginsenoside Rg3 high dose group and aminoguanidine group ( P <0.05).Conclusions Ginsenoside Rg3 can effectively promote the repair and healing of impaired diabetic wound.The various mechanisms of repair might be through improving skin pathology,regulating epidermal cell proliferation cycle and promoting angiogenesis.