花生叶提取物对电子束辐照后脂肪酶活性的保护作用

Protection of Peanut Leaf Extract on Lipase Activity Induced by Electron Beam Irradiation

[目的]研究不同剂量电子束辐照对脂肪酸的影响。[方法]通过不同花生叶提取物处理辐照下脂肪酶,探明花生叶提取物在脂肪酶活性中的保护作用。[结果]当5个电子束辐照剂量辐照脂肪酶,脂肪酶活力下降。当加入花生叶提取物后,不同类型花生叶提取物对辐照处理后的鲈鱼脂肪酶酶活性起到了一个促进作用,进一步SDS-PAGE分析发现,不同花生叶提取物对辐照后脂肪酶活性保护作用不相同。[结论]查询相关文献可知心型、脑型脂肪酸结合蛋白在鲈鱼脂肪酶中含量较高,说明该研究脂肪酸结合蛋白,主要集中在线粒体以及过氧化物酶体β氧化过程,此研究结果表明花生叶提取物对鲈鱼脂肪酶活性起到了一定保护作用。

[Objective]In order to study the effect of different doses of electron beam irradiation on fatty acids.[Method]The protective effect of peanut leaf extract on lipase activity was studied by treating different peanut leaf extracts under irradiation.[Result]Lipase activity decreased when 5 electron beam irradiation doses were used. When peanut leaf extracts were added, different types of peanut leaf extracts promoted lipase activity in irradiated perch. Further SDS-PAGE analysis showed that different peanut leaf extracts had different protective effects on lipase activity after irradiation.[Conclusion]The content of heart-type and brain-type fatty acid binding proteins in lipase of perch was higher than that of brain-type fatty acid binding proteins, which indicated that the fatty acid binding proteins in this study were mainly concentrated in mitochondria and the oxidation process of peroxisome beta. The stduy showed that peanut leaf extracts had a protective effect on lipase activity of perch.