砖红壤微生物总DNA的提取方法比较

Comparison of Extraction Methods for Total DNA of Microbes from Latosols

针对中国南方砖红土壤的特点,通过比较和优化土壤微生物总DNA的提取方法,获得较高质量的DNA,从而进行土壤细菌16S r DNA基因扩增和真菌I TS r DNA基因扩增。结果表明:方法一提取的土壤微生物DNA受到腐殖质等影响无法进行基因扩增;方法二提取的DNA可以进行土壤微生物基因组16S r DNA基因扩增,提取时间大大减少,但无法进行I TS r DNA基因扩增;方法三提取的DNA质量最好,可以进行细菌16S r DNA和真菌I TS r DNA基因组扩增。

According to the c haracteristics of latosols in southern China,extraction methods for soil microbial total DNA were compared and optimized to produce high quality total DNA s from the soil microbes in latosols,and the soil bacteria were identified by using the 16 S r DNA amplification and the soil fungi by using ITS r DNA.The results showed that the soil microbial DNA extracted by the first method could not be amplified by the ITS and 16 s primers because of the existence of humus and others.The DNA extracted by the second method could be used to amplify the 16 S r DNA gene of soil microbial genome with shorter extraction time,but the ITS r DNA gene amplification could not be performed.The extracted DNA by the third method was best in quality and could be used for bacterial and fungal genome amplification.