浙江省淳安县中华蜜蜂囊状幼虫病流行病学调查

Epidemiological investigation of Chinese sacbrood bee disease in honeybee (Apis cerana) in Chun'an County of Zhejiang Province, China

中蜂囊状幼虫病是制约中国中蜂养殖产业发展的主要病害。本研究在建立中蜂囊状幼虫病RT-PCR检测方法的基础上,对浙江省淳安县的中蜂进行中囊病的流行病学调查。结果显示:建立的RT-PCR检测方法对中囊病病毒特异性和重复性好,且灵敏度高,检测限为37 fg·mL^(-1)。用建立的RT-PCR检测方法检测淳安县17个乡镇共34个中蜂饲养点的307群工蜂样品,有13个乡镇中囊病病毒呈阳性,其中有6个乡镇阳性检出率超过50%。在中蜂饲养密集和有意蜂混养的地方,中囊病病毒阳性率较高。本研究明确了浙江省淳安县中囊病的流行情况,为该病害的预防和控制提供理论指导。

Chinese sacbrood bee disease is one of the serious diseases of Chinese honeybee,Apis cerana cerana.In this study,a RT-PCR method was developed to detect Chinese sacbrood bee virus(CSBV).Furthermore,an epidemiological investigation of CSBV in Chun an County of Zhejiang Province was conducted based on the new method.The results showed that the developed RT-PCR method had a good repeatability,a sharp specificity and a high sensibility,whose minimum detecting concentration was 37 fg·mL-1.The epidemiology of CSBV was investigated from 34 sample sites of 17 townships around Qiandao Lake area.The CSBV detection of 13 townships was positive,in which the infection rate was over 50%at 6 towns.The high infection rate of CSBV may be related to the high density and mixture honeybee species keeping.In conclusion,the epidemic situation of Chinese sacbrood bee disease in Chun an County was clarified and provided useful information for the disease control.