纳米碳与枯草菌对黄瓜幼苗生长及土壤环境的影响

Effect of nano-carbon sol and Bacillus subtilis on cucumber growth and soil environment

为筛选适宜黄瓜苗期生长的枯草芽孢杆菌浓度,以黄瓜幼苗为材料,设置纳米碳溶胶和枯草芽孢杆菌浓度双因素试验,纳米碳溶胶稀释倍数设0、350、650倍,枯草芽孢杆菌浓度为0、8×10~7、1.6×10~8 CFU·mL^(-1),分析枯草芽孢杆菌和纳米碳溶胶对黄瓜苗期长势、根系生长状况和土壤肥力的影响。结果表明,适宜浓度的枯草芽孢杆菌和纳米碳溶胶能够显著增强黄瓜幼苗的地上、地下部分生长,改善土壤状况。单施情况下,1.6×10~8 CFU·mL^(-1)枯草芽孢杆菌处理可以促进根系生长,显著降低土壤电导率(EC值)的同时提升土壤P含量,增强土壤酶活性,增加细菌、放线菌数量;650倍纳米碳溶胶处理可以显著促进植株叶片生长,提升土壤有机质含量和蔗糖酶活性,土壤放线菌数量增加57.36%。8×10~7 CFU·mL^(-1)枯草芽孢杆菌与650倍纳米碳溶胶共处理使株高相对生长率提高6.51%,显著促进根系生长,根系干质量、鲜质量、表面积、直径和体积均为处理中最高,比未添加菌剂与纳米碳的处理分别提高56.63%、57.14%、66.15%、56.55%、21.94%,降低土壤EC值,土壤中细菌数量是未添加菌剂与纳米碳的1.85倍,综合表现最优。

In order to screen the suitable concentration of Bacillus subtilis for cucumber seedling growth,the two-factor test of nano-carbon sol and Bacillus subtilis was carried out with cucumber seedlings which as the material.The dilution ratio of nano-carbon sol was set at 0,350 and 650 times,and the concentration of Bacillus subtilis was 0,8×10^7 and 1.6×10^8 CFU·mL^-1,respectively.The effects of Bacillus subtilis and nano-carbon sol on cucumber seedling growth,root growth and soil fertility were analyzed.The results showed that suitable concentration of Bacillus subtilis and nano-carbon sol could significantly enhance the growth of aboveground and underground parts of cucumber seedlings and improve soil conditions.Under single application,treatment with 1.6×10^8 CFU·mL^-1 Bacillus subtilis promoted root growth,significantly reduced soil EC value while increasing soil P content,enhancing soil enzyme activity,and increasing number of bacteria and actinomycetes.650 dilution multiple of nano-carbon sol significantly promoted plant leaf growth,soil organic matter content and sucrase activity,and increased the quantity of soil actinomycetes by 57.36%.The treatment of 8×10^7 CFU·mL^-1 Bacillus subtilis+650 dilution multiple of nano-carbon sol was optimal.Root fresh weight,dry weight,surface area,diameter and volume were the highest in these treatments,beyond the treatment without nano-carbon sol and bacillus subtilis 56.63%,57.14%,66.15%,56.55%and 21.94%.The relative growth rate of plant height was increased by 6.51%.The EC value of soil was reduced.The amount of bacteria in the soil was 1.85 times of the non-added nano-carbon sol and bacillus subtilis,and the overall performance was the best.