MYB基因沉默抑制子宫颈癌细胞侵袭、迁移及其作用机制探讨

Inhibition of invasion and migration of cervical cancer cells by MYB gene silencing and its mechanism

目的:探究沉默转录因子MYB对子宫颈癌细胞侵袭、迁移的影响以及可能的作用机制。方法:将携带MYB目的片段的siRNA转染入人子宫颈癌HeLa细胞中。实验分为对照组、阴性对照组和siRNA-MYB组。采用荧光定量PCR(qPCR)和蛋白质印迹法(Western Blot)检测转染效果。细胞划痕实验和Transwell法检测细胞的迁移、侵袭能力。Western Blot检测各组细胞中基质金属蛋白酶-2(MMP-2)、钙黏蛋白E(E-cadherin)、钙黏蛋白N(N-cadherin)、波形蛋白(Vimentin)水平。结果:与对照组相比,阴性对照组细胞中MYB的表达量、细胞侵袭、迁移均无显著变化,差异不具有统计学意义;siRNA-MYB组细胞中MYB的表达量显著降低(P<0.05),沉默MYB显著抑制子宫颈癌细胞的侵袭、迁移(P<0.05),并下调细胞中MMP-2、N-cadherin、Vimentin蛋白的表达量(P<0.05),上调E-cadherin蛋白的表达量(P<0.05)。结论:沉默宫颈癌细胞中MYB的表达量可通过抑制EMT、下调MMP-2蛋白水平,从而降低细胞的侵袭、迁移能力。

Objective:To investigate the effect of silencing MYB on the invasion and migration of cervical cancer cells and its possible mechanism.Methods:siRNAs was transfected into human cervical cancer HeLa cells to silence the expression of MYB gene.The experiments were divided into control group,negative control group and siRNA-MYB group.Transfection efficiency was examined by qPCR and Western Blot.The migration and invasion of cells were analyzed by cell scratch test and Transwell assay.The protein levels of matrix metalloproteinase-2(MMP-2),E-cadherin,N-cadherin and Vimentin were examined by Western Blot.Results:Compared with the control group,there was no significant change in the expression of MYB,the invasion and migration of cells in the negative control group.The expression of MYB in the siRNA-MYB group was significantly lower than that in the control group(P<0.05).Silencing MYB significantly inhibited the invasion and migration of cervical cancer cells(P<0.05),down-regulated the expression of MMP-2,N-cadherin,and Vimentin proteins(P<0.05),and up-regulated the expression of E-cadherin protein(P<0.05).Conclusion:The expression of MYB can be inhibited the invasion and migration ability of cervical cancer cells by inhibiting EMT and down-regulating the level of MMP-2 protein,thus reducing the invasion and migration of cells.