光合-厌氧混合菌群生物共发酵产氢动力学研究

Dynamic Characteristics of Co-fermentative Hydrogen Production by Mixed Photosynthetic and Anaerobic Consortium

以光合、厌氧细菌混合菌群为对象,研究了混合菌群共发酵产氢过程中产氢动力学特性,建立了混合菌群生物共发酵产氢过程中关于菌体质量浓度、底物利用及产氢量的动力学模型。将光照因素引入混合菌群产氢动力学模型中,采用同伦摄动法(HPM)对非线性动力学模型进行求解,得到了混合菌群共发酵产氢过程中菌体质量浓度、底物利用及产氢量的动力学模型。通过与实验数据对比,模型与实验数据基本一致,能够很好地反映出共发酵产氢过程中产氢参数的变化趋势。对建立的3个动力学模型的动力学参数的相互关系及其敏感性进行了分析,研究发现动力学参数中最大比生长速率对模型结果的影响最大,最大比生长速率对菌体质量浓度影响的变化量达到79%,对底物质量浓度影响的变化量达到118%,对产氢量影响的变化量达到98. 4%。

Aiming to combine the advantage of photo-fermentation and dark-fermentation for biohydrogen production,the mixed photosynthetic and anaerobic bacteria consortium(MPAC)was used to study the co-fermentative hydrogen production.The kinetic characteristics of co-fermentative hydrogen production by MPAC were studied.The kinetic models of cell growth,substrate utilization and hydrogen production of co-fermentative hydrogen production by MPAC were established.The illumination factor was firstly introduced into the kinetic model of bio-hydrogen production.The nonlinear differential equation was solved by homotopy perturbation method(HPM)and the kinetic models were obtained.By comparing with the experimental data,the model can be consistent with the experimental data,which can well reflect the change of biomass concentration,substrate concentration and hydrogen production in the process of co-fermentative hydrogen production.The relationship between the kinetic parameters and the sensitivity of the three established kinetic models were analyzed.The results showed that the maximum specific growth rate of the kinetic models had the biggest influence on the results,and the change of the cell growth reached 79%,the change of substrate concentration reached 118%,and the change of hydrogen produced reached 98.4%.The three established models were suitable for describing the kinetic characteristics of biohydrogen production.