玉米骨干自交系对除草剂苯磺隆和甲咪唑烟酸的敏感性差异

The Sensitivity Differences of Maize Backbone Inbred Lines to the Herbicides,Tribenuron-methyl and Imazapic

为了在培育玉米抗除草剂突变体过程中选择合适的受体材料,以7个玉米骨干自交系C92、京724、京2416、B73、郑58、HZ178、178为材料,研究不同自交系对苯磺隆和甲咪唑烟酸的敏感性差异。测定了不同浓度除草剂处理下各自交系的初生根长抑制率、幼苗生长抑制率和乙酰乳酸合成酶(ALS)活性,结果显示,随着2种除草剂浓度的增加,各自交系的初生根长抑制率和幼苗生长抑制率明显提高,且各自交系的ALS活性存在不同程度下降趋势。7个自交系对2种除草剂的敏感性均为C92>京724>京2416>B73>郑58>HZ178>178,各自交系均对甲咪唑烟酸更敏感。综上所述,自交系C92、京724、京2416相比B73、郑58、178、HZ178对苯磺隆和甲咪唑烟酸更加敏感,适合作为培育抗除草剂突变体的受体材料。

In order to select the right receptor material during the cultivation of herbicide resistant mutants,the sensitivity of various maize inbred lines to tribenuron-methyl and imazapic was studied using seven maize backbone inbred lines,C92,Jing 724,Jing 2416,B73,Zheng 58,HZ178 and 178.We measured the inhibition rates of primary root length,seedling growth and acetolactate synthase(ALS)activity of each inbred line under different herbicide concentration.The results showed that the inhibition rates of primary root length and seedling growth of each inbred line increased obviously with the increase of the treatment concentration of two herbicides.And the ALS activity of each inbred line decreased to different degrees.The sensitivities of seven inbred lines under two herbicides were:C92>Jing 724>Jing 2416>B73>Zheng 58>HZ178>178.All the inbred lines were more sensitive to imazapic than tribenuron-methyl.To sum up,compared with B73,Zheng 58,178 and HZ178,the inbred lines C92,Jing 724 and Jing 2416 are more sensitive to tribenuron-methyl and imazapic,and suitable as receptor materials for cultivation of anti-herbicide mutant.