摘要
为研究复合植物精油(OCT)对脂多糖(LPS)刺激断奶仔猪肠道结构和抗氧化能力的影响,本试验选取28日龄左右健康的杜×长×大三元杂交仔猪24头,按照体重相近原则随机分为3个处理组:对照组、LPS组和OCT组。对照组和LPS组仔猪饲喂基础日粮,OCT组仔猪饲喂基础日粮+50 mg/kg OCT。试验期21 d。于试验第21天,LPS组和OCT组仔猪注射LPS(100μg/(kg·BW)),对照组仔猪注射等量生理盐水。LPS或生理盐水注射后3 h采血;6 h后,屠宰全部仔猪取肠道样品测定有关指标。结果表明,与对照组相比,LPS组仔猪空肠绒毛高度/隐窝深度有降低趋势(P>0.05),回肠绒毛高度及绒毛高度/隐窝深度显著降低(P<0.05);血浆过氧化氢酶(CAT)活性、血浆和空肠超氧化物歧化酶(SOD)活性显著降低(P<0.05),空肠诱导型一氧化氮合酶(iNOS)活性、血浆和回肠H_2O_2含量显著上升(P<0.05)。与LPS组相比,OCT组仔猪空肠绒毛高度、空肠绒毛高度/隐窝深度和回肠绒毛高度显著提高(P<0.05),回肠绒毛高度/隐窝深度有提高趋势(P>0.05);血浆CAT、空肠SOD活性显著提高(P<0.05),血浆和回肠H_2O_2含量显著下降(P<0.05)。以上结果表明,日粮中添加50 mg/kg OCT可以在一定程度上缓解由LPS刺激引起的仔猪氧化应激,改善仔猪肠道结构。
In order to investigate the effect of compound plant essential oil (OCT) on structure of intestinal mucosa and antioxidant capacity of weaned piglets challenged with lipopolysaccharid (LPS),twenty-four weaned piglets (28-day- old,Duroc×Landrace×Yorkshire) were randomly assigned into 3 groups:Control group,LPS group and OCT group.Piglets in the control and LPS groups were fed a basal diet,and piglets in the OCT group received the basal diet+50 mg/kg OCT.On the 21th day of the trail,piglets in the LPS and OCT groups were injected intraperitoneally with LPS (100 μg/(kg·BW)),and piglets in the control group were injected with the same volume of physiological saline.Venous blood samples were obtained at 3 h post LPS or saline injection,all piglets were killed to obtain the intestinal mucosa for analysis at 6 h post LPS or saline injection.Compared with the control group,LPS stimulation had a tendency to decrease villus height/crypt depth in jejunal mucosa (P>0.05),it could cause a significant reduction in villus height and villous height/crypt depth in ileal mucosa;the activity of CAT and SOD in plasma,and SOD in jejunum mucosa were decreased significantly (P<0.05),it also increased the activity of iNOS in jejunum mucosa,the content of H2O2 in plasma and ileum mucosa significantly(P<0.05).Compared with the LPS group,adding OCT could increase villus height and villus height/crypt depth in jejunal mucosa,villus height in ileal mucosa significantly (P<0.05),and villus height/crypt depth in ileal mucosa had a tendency to increase (P>0.05);The activity of CAT in plasma and SOD in jejunum mucosa were also increased significantly (P<0.05),the content of H2O2 in plasma and ileum mucosa were decreased significantly (P<0.05).In conclusion,the addition of 50 mg/kg OCT to the diet could alleviate the oxidative stress induced by LPS challenge to a certain extent and improve the intestinal structure of piglets.
作者
宋转
赵广宇
董毅
纪昌正
李思源
王蕾
易丹
侯永清
SONG Zhuan;ZHAO Guangyu;DONG Yi;JI Changzheng;LI Siyuan;WANG Lei;YI Dan;HOU Yongqing(Engineering Research Center of Feed Protein Resources on Agricultural By-products,Ministry of Education,Wuhan Polytechnic University,Wuhan 430023,China;Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第3期684-689,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家重点研发计划课题(2016YFD0501210)
关键词
复合植物精油
断奶仔猪
脂多糖
肠黏膜
肠道形态结构
抗氧化能力
compound plant essential oil
weaned piglets
lipopolysaccharide
intestinal mucosa
intestinal morphology
antioxidant capacity