复合植物精油对脂多糖刺激断奶仔猪肠道形态结构和抗氧化能力的影响

Effect of Compound Plant Essential Oils on Structure of Intestinal Mucosa and Antioxidant Capacity of Weaned Piglets Challenged with Lipopolysaccharide

为研究复合植物精油(OCT)对脂多糖(LPS)刺激断奶仔猪肠道结构和抗氧化能力的影响,本试验选取28日龄左右健康的杜×长×大三元杂交仔猪24头,按照体重相近原则随机分为3个处理组:对照组、LPS组和OCT组。对照组和LPS组仔猪饲喂基础日粮,OCT组仔猪饲喂基础日粮+50 mg/kg OCT。试验期21 d。于试验第21天,LPS组和OCT组仔猪注射LPS(100μg/(kg·BW)),对照组仔猪注射等量生理盐水。LPS或生理盐水注射后3 h采血;6 h后,屠宰全部仔猪取肠道样品测定有关指标。结果表明,与对照组相比,LPS组仔猪空肠绒毛高度/隐窝深度有降低趋势(P>0.05),回肠绒毛高度及绒毛高度/隐窝深度显著降低(P<0.05);血浆过氧化氢酶(CAT)活性、血浆和空肠超氧化物歧化酶(SOD)活性显著降低(P<0.05),空肠诱导型一氧化氮合酶(iNOS)活性、血浆和回肠H_2O_2含量显著上升(P<0.05)。与LPS组相比,OCT组仔猪空肠绒毛高度、空肠绒毛高度/隐窝深度和回肠绒毛高度显著提高(P<0.05),回肠绒毛高度/隐窝深度有提高趋势(P>0.05);血浆CAT、空肠SOD活性显著提高(P<0.05),血浆和回肠H_2O_2含量显著下降(P<0.05)。以上结果表明,日粮中添加50 mg/kg OCT可以在一定程度上缓解由LPS刺激引起的仔猪氧化应激,改善仔猪肠道结构。

In order to investigate the effect of compound plant essential oil (OCT) on structure of intestinal mucosa and antioxidant capacity of weaned piglets challenged with lipopolysaccharid (LPS),twenty-four weaned piglets (28-day- old,Duroc×Landrace×Yorkshire) were randomly assigned into 3 groups:Control group,LPS group and OCT group.Piglets in the control and LPS groups were fed a basal diet,and piglets in the OCT group received the basal diet+50 mg/kg OCT.On the 21th day of the trail,piglets in the LPS and OCT groups were injected intraperitoneally with LPS (100 μg/(kg·BW)),and piglets in the control group were injected with the same volume of physiological saline.Venous blood samples were obtained at 3 h post LPS or saline injection,all piglets were killed to obtain the intestinal mucosa for analysis at 6 h post LPS or saline injection.Compared with the control group,LPS stimulation had a tendency to decrease villus height/crypt depth in jejunal mucosa (P>0.05),it could cause a significant reduction in villus height and villous height/crypt depth in ileal mucosa;the activity of CAT and SOD in plasma,and SOD in jejunum mucosa were decreased significantly (P<0.05),it also increased the activity of iNOS in jejunum mucosa,the content of H2O2 in plasma and ileum mucosa significantly(P<0.05).Compared with the LPS group,adding OCT could increase villus height and villus height/crypt depth in jejunal mucosa,villus height in ileal mucosa significantly (P<0.05),and villus height/crypt depth in ileal mucosa had a tendency to increase (P>0.05);The activity of CAT in plasma and SOD in jejunum mucosa were also increased significantly (P<0.05),the content of H2O2 in plasma and ileum mucosa were decreased significantly (P<0.05).In conclusion,the addition of 50 mg/kg OCT to the diet could alleviate the oxidative stress induced by LPS challenge to a certain extent and improve the intestinal structure of piglets.