德州驴乳清蛋白差异蛋白质组学研究

Study of Differential Proteomics of Dezhou Donkey Whey Proteins

驴奶的营养成分比例与母乳相近,是牛奶的最佳替代乳品。为研究影响驴产奶量的分子机制,采用非标记质谱法对不同产奶量德州驴的乳清蛋白进行了定量分析。结果表明:德州驴奶共鉴定出216个乳清蛋白,其中19个在高产样本中存在显著差异(P<0.05);在这些蛋白质中,有16个上调,3个下调。对差异表达蛋白(DEPs)生物信息学分析表明,大多数DEPs参与了内质网蛋白合成、雌激素信号通路、孕酮介导卵母细胞成熟和PI3K-Akt信号通路。文章首次对高产驴和低产驴奶样中乳清蛋白质谱进行分析,并确定了与德州驴产奶量性状相关的分子机制的候选蛋白质,为高产奶驴的选育提供了生物标记。

Donkey milk has a similar proportion of nutrients to breast milk and is the best substitute for milk.In this study,label-free mass spectrometry analysis was conducted to quantitatively identify the whey proteins expressed in Dezhou donkey of different milk yields.Results revealed that a total of 216 whey proteins were identified,and 19 of them showed significant differences in high-milk-yield samples.Of these proteins,16 were upregulated and 3 were downregulated.Differentially expressed proteins(DEPs)were subjected to intensive bioinformatic analysis.The majority of DEPs participated in protein processing in endoplasmic reticulum,estrogen signaling pathway,progesterone-mediated oocyte maturation,and PI3K-Akt signaling pathway.This study analyzed the whey protein profile of high milk yield donkey and low milk yield donkey and to identify candidate proteins that related to the molecular mechanism of the yield traits of Dezhou donkey.This study provided the biomarkers for the selection of high-milk-yielding donkey.