microRNA-146a单核苷酸多态性与大肠癌遗传易感性的关联研究

Association of a MiRNA-146a Polymorphism with in Colorectal Cancer in Chinese Han Population

目的分析microRNA-146a单核苷酸多态性与大肠癌遗传易感性的相关性。方法通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,对251例中国汉族大肠癌患者(实验组)和280例年龄和地区匹配的正常对照人群(对照组)进行相关性研究,采取实时定量聚合酶链反应(RT-PCR)法检测携带不同基因型患者大肠癌组织中成熟miR-146a表达情况。结果两组GG、GC、CC基因型分布及G、C等位基因频率存在显著性差异(P <0. 05); CC基因型与GC+GG基因型在无肿瘤家族史人群中存在显著性差异(P <0. 05)。结论 miR-146a单核苷酸多态性位点rs2910164与中国汉族人群大肠癌的易感性有关,且可影响大肠癌患者体内成熟miR-146a的表达。

Objective To analyse Association of a MiRNA-146a Polymorphism with in Colorectal Cancer in Chinese Han Population.Methods Blood samples were collected from 251 Chinese colorectal cancer patients(experimental group)and 280 controls(control group).All samples were genotyped for the rs2910164 SNP of miR-146a using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Tissue samples of colorectal cancer were collected from 30 Chinese colorectal cancer patients.The association between the rs2910164 SNP and miR-146a expression in colorectal cancer was analyzed by real-time quantitative polymerase chain reaction(RT-PCR).Results There were significant differences in the distribution of GG,GC,CC genotypes and the frequency of G,C alleles between the two groups(P<0.05);Significant differences was obversed between CC genotype and GC+GG allele carrier in the subgroup of individuals without a family history of cancer(P<0.05).Conclusion There is a significant association of a miRNA-146a polymorphism with in colorectal cancer in Chinese Han population.The expression of miR-146a in colorectal cancer was significant influenced by miRNA-146a polymorphism.