肝再生过程中胆汁酸对FXR的影响

Effect of cholic acid juice on X receptor in liver regeneration

目的分析胆酸汁在肝再生中对法尼基衍生物X受体(FXR)的影响。方法选取60只SD大鼠,分别建立肝切除30%、50%、70%的大鼠模型,每组20只;并同时根据喂养方式的不同分为Ⅰ组(葡萄糖喂养)和Ⅱ组(胆汁酸喂养),每组10只,共计6个组,分别为AⅠ、AⅡ、BⅠ、BⅡ、CⅠ、CⅡ。在喂养24h、48h、72h后检测每组小鼠的肝再生率、FXR及ALB表达水平。结果Ⅱ组大鼠在24h、48h、72h时肝再生率均明显高于Ⅰ组(均P<0.05),Ⅰ组24h、48h、72h肝再生率最高的为AⅠ,其次为BⅠ、CⅠ,Ⅱ组24h、48h、72h肝再生率最高的为AⅡ,其次为BⅡ、CⅡ。Ⅱ组大鼠在24h、48h、72h的FXR表达水平均明显高于Ⅰ组(均P<0.05),Ⅰ组24h、48h、72h时FXR表达水平最高的为AⅠ,其次为BⅠ、CⅠ,Ⅱ组24h、48h、72h的FXR表达水平最高为AⅡ,其次为BⅡ、CⅡ。Ⅱ组大鼠在24h、48h、72h的ALB表达水平均明显高于Ⅰ组(均P<0.05),Ⅰ组24h、48h、72h的ALB表达水平最高为AⅠ,其次为BⅠ、CⅠ,Ⅱ组24h、48h、72h的ALB表达水平最高为AⅡ,其次为BⅡ、CⅡ。结论胆汁酸能够通过提高FXR表达水平从而加速肝再生和白蛋白的合成能力。

Objective To analyze the effect of cholic acid juice on the X receptor (FXR) in liver regeneration. Methods 60 SD rats were selected to establish rat models with excision of 30%, 50%, and 70% of the livers, 20 rats for each model. According to the different feeding ways, all the three models were divided into group Ⅰ(glucose feeding) and group Ⅱ(bile acid feeding), 10 for each group, with a total of six groups (A Ⅰ, A Ⅱ, B Ⅰ, B Ⅱ, C Ⅰ, and C Ⅱ). The liver regeneration rate and expression levels ofFXR and ALB in each group were detected after feeding 24, 48, and 72 h. Results After feeding 24, 48, and 72 h, the liver regeneration rates and levels of FXR and ALB were higher in group Ⅱ than in group Ⅰ(all P < 0.05);those in group A Ⅰ were higher than those in group B Ⅰ, and those in group B Ⅰ than those in group C Ⅰ;and those in group A Ⅱ were higher than those in group B Ⅱ, and those in group B Ⅱ than those in group C Ⅱ. Conclusion Bile acid can accelerate the synthesis of liver regeneration and albumin increasing FXR expression level.