Shh和Foxm1在宫颈癌组织中的表达及意义

Expressions of Shh and Foxm1 in cervical cancer and its’ significance

目的探讨Shh和Foxm1在宫颈癌组织中的表达及意义。方法收集宫颈组织标本152例,其中正常宫颈组织40例,宫颈上皮内瘤变(CIN)Ⅰ~Ⅱ30例、CINⅢ35例,宫颈癌47例。采用免疫组化法检测标本中Shh和Foxm1蛋白表达,分析两者阳性表达率与临床特征的关系及两者在宫颈癌组织中表达的关系。结果CINⅢ组织Shh和Foxm1阳性表达率分别为57.1%、54.3%,宫颈癌59.6%、61.7%,差异均无统计学意义(均P>0.05),但均明显高于正常宫颈组织(15.0%、12.5%)和CINⅠ~Ⅱ组织(30.0%、26.7%)(均P<0.05)。中-低分化、有淋巴结转移、Ⅲ~Ⅳ分期Shh和Foxm1阳性表达率分别为77.3%和86.4%、86.7%和93.3%、73.7%和84.2%,明显高于高分化(44.0%、40.0%)、无淋巴结转移(46.9%、43.8%)、Ⅰ~Ⅱ分期(50.0%、46.4%)(均P<0.05)。Spearman等级相关性分析结果显示,宫颈癌组织中Shh表达与Foxm1表达呈正相关性(r=0.542,P<0.05)。结论Shh、Foxm1在宫颈癌组织异常高表达,两者呈正相关,可能通过Shh信号通路参与了宫颈癌的发生和进展过程,这为宫颈癌的分子靶向药物治疗提供可能的理论依据,也可能为宫颈癌的早期筛查诊断及治疗提供了重要的生物学指标。

Objective To investigate the expressions and significance of Shh and Foxm1 in cervical cancer. Methods 152 cervical tissue specimens were collected, including 40 normal cervical tissue,30 cases of cervical intraepithelial neoplasia (CIN Ⅰ-Ⅱ), 35 cases of CIN Ⅲ, and 47 cases of cervical cancer. The expressions of Shh and Foxm1 in the specimens were detected by immunohistochemical method. The relationships between the positive expression rate and clinical characteristics as well as the expressions of Shh and Foxm1 in the cervical cancer tissue were analyzed. Results The positive expression rates of Shh and Foxm1 in the CIN Ⅲ tissue were 57.1% and 54.3%, and those in the cervical cancer tissue were 59.6% and 61.7%, with no staitiscal differences (both P > 0.05);but they were significantly higher than those in the normal cervical tissue (15.0% and 12.5%) and those in the CIN Ⅰ-Ⅱ tissue (30.0% and 26.7%)(all P < 0.05). The positive expression rates of SHH and Foxm1 were 77.3% and 86.4% in the moderately and poorly differentiated tissue, 86.7% and 93.3% in the lymphatic metastasis, and 73.7% and 84.2% in the Ⅲ-Ⅳ, which were significantly higher than those in high differentiation (44.0% and 40.0%), those in no lymph node metastasis (46.9% and 43.8%), and those in the Ⅰ-Ⅱ(50.0% and 46.4%)(all P < 0.05). Spearman grade correlation analysis showed that Shh expression positively correlated with Foxm1 expression in cervical cancer (r=0.542, P < 0.05). Conclusions Shh and Foxm1 are highly expressed in cervical cancer tissue. They are positively correlated. They may participate in the occurrence and progression of cervical cancer through Shh signaling pathway, which provides a possible theoretical basis for molecular targeted drug therapy of cervical cancer, and may also provide an important biological basis for early screening, diagnosis, and treatment of cervical cancer.