摘要
过去的二十年中,以电子捕获裂解为代表的离子反应技术,极大地推动了气相自由基离子化学的研究.该领域的发展是现代质谱的前沿问题,同时也是后基因组时代生物分析的重要技术手段.总结了电子捕获裂解的研究进展,并对其产生、裂解规律以及机理的研究进行了详细的综述.
In the past twenty years, our knowledge on gas phase radical ion chemistry has been significantly improved due to the development of electron capture dissociation. Combined with soft ionization method, it has shown the technique can provide novel fragment ions for the structural elucidation of biomolecules, especially for protein characterization. This review aims to introduce fundamental aspects of electron capture dissociation mass spectrometry, as well as its applications in the analysis of biomolecules.
作者
汪泽
黄漪铃
任娟
陈相峰
陈德华
Wang Ze;Wong Y.-L.Elaine;Ren Juan;Chen Xiangfeng;Chan T.-W.Dominic(Key Laboratory of Fundamental and Applied Research of Traditional Chinese Medicine, Zunyi Medical University at Zhuhai Campus, Zhuhai 519090;Department of Chemistry, The Chinese University of Hong Kong, Hong Kong SAR;Instrumental Analysis and Testing Center, Shandong Academy of Science, Jinan 250014)
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2019年第2期130-142,共13页
Acta Chimica Sinica
基金
国家自然科学基金(No.21205071)
香港研究资助局项目(Nos.3132667,4053152)
山东省自然科学基金(No.ZR2017MB011)资助~~
关键词
质谱
电子捕获裂解
气相离子化学
蛋白质分析
mass spectrometry
electron capture dissociation
gas phase ion chemistry
protein characterization
