一株甘蓝内生枯草芽孢杆菌16S rDNA序列的克隆与分析

Cloning and Analysis of 16S rDNA Sequence of an Endophytic Bacillus subtilis Strain Isolated from Cabbage

台州学院生命科学学院实验室前期从甘蓝健康植株中分离到一株内生细菌GL06,为甘蓝内生枯草芽孢杆菌GL06的功能研究和生产应用奠定基础,利用PCR法克隆其16S rDNA序列,并进行生物信息学分析。结果表明:以27F/1492R为PCR引物,扩增到长度为1 510bp的16S rDNA序列,序列的GC含量为54%;GL06 16S与枯草芽孢杆菌16S的相似性最高,达99%,仅存在3个碱基差异;GL06与枯草芽孢杆菌、解淀粉芽孢杆菌的系统发育树聚为一组。

The 16S rDNA sequence and biological information of an endophytic Bacillus subtilis strain GL06 isolated from healthy cabbage plants by College of Life Sciences,Taizhou University was cloned and analyzed by PCR to lay the foundation for its function research and production application.Result: The length and GC content of the 16S rDNA sequence amplified by using 27F/1492R as a primer are 1 510 bp and 54% respectively.The 16S similarity between GL06 strain and B. subtilis is up to 99% and there is a three-base difference only.The phylogenetic tree of GL06 strain, B.subtilis and Bacillus amyloliquefaciens can be divided into the same group.