微小RNA-1266在房颤及心力衰竭患者中的表达

Expression and Significance of microRNA-1266 in Patients with Atrial Fibrillation and Heart Failure

目的:探究微小RNA-1266在房颤(AF)及心力衰竭(HF)患者中的表达及意义。方法:选取41例AF患者作为AF组,38例HF患者作为HF组,41例HF并发AF患者作为HF+AF组,同时选取同期体检健康者30例作为对照组;收集4组受试者年龄、性别、高血压史、糖尿病史及吸烟情况,记录3组患者β受体阻滞剂的使用情况;比较入院时或体检时4组受试者收缩压(mm Hg)、舒张压(mm Hg)、体质量指数(kg/m^2)、肌酐、血糖、总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白、N端前脑钠素(NT-proBNP)、左心室射血分数(LVEF)、左心房直径(LAD)及心胸比;采用Real-time PCR检测入院或体检时4组受试者血清miR-1266水平,使用Pearson检验和多元线性回归分析评价患者上述各指标与血清miR-1266表达的相关性。结果:与对照组比较,3组患者NT-proBNP水平显著升高(P <0. 05),HF组和HF+AF组又显著高于AF组(P <0. 05);对照组LVEF明显高于3组患者,AF组的LVEF又显著高于HF及HF-AF组(P <0. 05); 3组患者的LAD均显著大于对照组(P <0. 05); miR-1266在AF、HF、HF+AF组患者血清表达较对照组显著升高(P <0. 05),HF+AF组又显著高于AF组及HF组(P <0. 05); Pearson检验结果显示,血清miR-1266表达与AF+HF组LVEF呈负相关(r=-0. 360,P=0. 019),与Ig(NT-proBNP)、LAD、心胸比正相关(r=0. 779、0. 510、0. 588,P <0. 001),血清miR-1266水平与HF-AF患者的年龄呈正相关(r=0. 321,P <0. 001);多元线性回归分析发现血清miR-1266的表达与Ig(NTpro BNP)、心胸比呈正相关(P <0. 05),与LVEF呈负相关(P <0. 05)。结论:miR-1266血清水平可以作为评估AF和HF严重程度的潜在生物标志物。

Objective:To explore the expression and significance of microRNA-1266 in patients with atrial fibrillation and heart failure.Methods:41 Patients with atrial fibrillation(AF)as AF group,38 patients with heart failure(HF)as HF group,and 41 HF complicated with AF(HF+AF)patients as HF+AF group;30 healthy people on physical check purpose were chosen as control group.Following data were collected from all groups:gender,age,hyper-blood pressure history,diabetes history and smoking conditions;recordingβblockers usage condition of patients groups;comparing following value from all groups when they were hospitalized or from physical check:SBP(mmHg),DBP(mmHg),BMI(kg/m 2),CRE,glucose,total cholesterol,TG,LDL,HDL,NT-proBNP,LVEF,LAD and CTR.Real-time PCR was adopted to detect serum miR-1266 levels when all groups subjects were hospitalized.Pearson test and multiple linear regression analysis were used to evaluate the correlation between clinical pathological parameters and serum miR-1266 expression.Results:Comparing with control group,NT-proBNP level of three experiment groups significantly increased(P<0.05),the data of HF group and HF+AF group was obviously higher than that of AF group(P<0.05);LVEF of control group was also significantly higher than the other 3 groups,LVEF of AF group was obviously higher than HF and HF+AF group(P<0.05);LAD of 3 experiment groups was obviously higher than that of control group(P<0.05).The serum expression of miR-1266 in AF,HF and HF+AF patients was significantly higher than that of control group(P<0.05);the expression of miR-1266 in HF+AF group was significantly higher than that of AF group and HF group(P<0.05).Pearson test indicated that serum miR-1266 expression and LVEF of AF+HF group was negatively correlated(r=-0.360,P=0.019),and positively related with Ig(NT-proBNP),LAD and CTR(r=0.779,0.510,0.588,P<0.001);serum miR-1266 level was positively correlated with the age of HF-AF patients(r=0.321,P<0.001).Multiple linear regression analysis showed that the expression level of serum miR-1266 was positively correlated with Ig(NT-proBNP)and cardiothoracic ratio(P<0.05).Conclusion:miR-1266 serum level can be used as potential biomarkers for assessing the severity of AF and HF.