拟南芥ABA受体与UGT71B6的相互作用研究

The research on the interaction between the UGT71B6 and ABA receptors in Arabidopsis

为进一步分析ABA尿苷二磷酸葡萄糖基转移酶UGT71B6参与植物抗逆反应的具体机制,本研究通过体外酵母双杂交实验与GST-Pull down实验、体内双分子荧光互补实验(BIFC)分析了ABA受体RCARs与UGT71B6之间的相互作用关系.实验结果显示ABA受体RCAR12、RCAR13与UGT71B6,在体内、体外均存在直接的相互作用.研究表明UGT71B6的生理功能与ABA受体之间存在重要联系, UGT71B6可能通过ABA受体的介导参与了植物逆境应答反应.实验结果也为初步探究ABA受体是否参与ABA稳态调节过程提供一定参考.

In order to investigate whether ABA receptors participate in ABA homeostatic regulation mediated by UGT71B6 and analyze the specific mechanisms of UGT71B6's involvement in plant stress responses, we analyzed the interaction between RCARs and UGT71B6 by yeast two-hybrid assay, BIFC, and GST-pull down assay. The results indicated that UGT71B6 interacted with RCAR12/13 in vivo and in vitro. Thus, it is likely that the mechanism of UGT71B6 participating in plant abiotic responses is related to its interaction with ABA RCARs. The experimental results also provide some references for the role studying of ABA receptors in the ABA homeostasis regulation process.