拟南芥E3泛素连接酶AtARRE与ABI5相互作用分析

Analysis of the interaction between E3 ubiquitin ligase AtARRE and ABI5 in Arabidopsis thaliana

为了阐述AtARRE与ABA信号通路关键转录因子ABI5的作用,本研究采用原生质体瞬间表达系统探究了AtARRE蛋白的亚细胞定位,证明AtARRE蛋白定位于细胞核.随后,采用酵母双杂交和GST-Pull down技术分析了AtARRE与ABI5在体外的相互作用,证明AtARRE与ABI5在体外存在相互作用.最后,本研究采用双分子荧光互补实验进一步分析AtARRE与ABI5在体内的相互作用,结果表明共表达AtARRE与ABI5在植物体内存在相互作用.这些结果共同表明AtARRE可能参与了ABI5介导的植物对逆境的响应.

Based on the study of AtARRE at the gene and protein levels, this paper describes its role with the key transcription factor ABI5 of the ABA signaling pathway. In this study, a protoplast transient expression system was used to explore the subcellular localization of AtARRE protein, and the result demonstrated that AtARRE localized in the nucleus. Subsequently, the interaction between AtARRE and ABI5 was analyzed by yeast two-hybrid and GST-Pull down techniques. Our analysis demonstrated that AtARRE interacted with ABI5 in vitro . At last, analysis by bimolecular fluorescence complementation experiments further demonstrated the interaction between AtARRE and ABI5, indicating that the co-expression of AtARRE and ABI5 had interaction in vivo . These results indicate that AtARRE may be involved in ABI5-mediated plant response to stress.