锦绣杜鹃叶抗菌活性及其有效部位研究

ANTIBACTERIAL ACTIVITIES OF RHODODENDRON PULCHRUM LEAVES AND ITS ACTIVE FRACTION

为了研究锦绣杜鹃叶的抗菌活性,本实验采用了甲醇冷浸法、乙醇回流法和微波萃取法分别对锦绣杜鹃叶的有效成分进行提取,并采用微孔板稀释法测试提取物对多种阳性菌、阴性菌和耐药菌的抑制活性,通过紫外分光光度法测定了各萃取物的总黄酮含量。此外,为了获取锦绣杜鹃叶抗菌作用的有效部位,本实验将提取物分别用石油醚、乙酸乙酯和水萃取得到不同极性的萃取物组分,比较其抑菌活性。结果显示:乙醇回流法获得的粗提物的抗菌活性最佳,对铜绿假单胞27853的MIC值达到了0.512 mg/mL;各萃取组分中,水萃取组分和乙酸乙酯萃取组分的抗菌效果最好,三种提取方法得到的水萃取组分对金黄色葡萄球菌26003均达到1.024mg/mL,微波萃取法得到的乙酸乙酯萃取组分对铜绿假单胞菌27853的MIC值达到了0.512 mg/mL。本研究证实了锦绣杜鹃叶具有较好的的抗菌活性,其抗菌作用的有效部位为水层提取部位,为锦绣杜鹃叶在抗菌药物和相关产品研究开发方面提供了实验依据。

To investigate the antibacterial activities of the extraction of Rhododendron pulchrum leaves and its different extraction methods for antibacterial active substances. Extraction methods including methanol soaking, ethanol refluxing and microwave extracting were carried out. The antibacterial activities of the extracted components were evaluated by microporous plate dilution test using various positive bacteria, negative bacteria and antibiotic resistant bacteria as the tested bacteria. The total flavonoid content of different extractions were determined by ultraviolet spectroscopy. Additionally, the crude extract was extracted with petroleum ether, ethyl acetate and water respectively to get three different polar components, which were also subjected to the antibacterial test. The resdlts indicated that The ethanol refluxing method was superior to the other extract methods in the aspect of antibacterial activity. Furthermore, the water phase and the ethyl acetate phase had better antibacterial effects than the petroleum ether phase, the water phases in three methods all had antibacterial activities against Staphylococcus aureus 26003 with a MIC value of 1.024 mg/Ml. The ethyl acetate phase from microwave extracting expressed antibacterial activities against Pseudomonas aureus 27853 with a MIC value of 0.512 mg/mL. R. pulchrum leaves exhibited antibacterial activities, and the water extract was the effective antibacterial ingredients. It provides experimental evidences for the research and development of R. pulchrum leaf as antibacterial agents or related products.