淫羊藿苷通过Wnt/β-catenin信号通路对卵巢癌细胞CAOV3增殖的影响

Effects of Icariin on the Proliferation of Ovarian Cancer CAOV3 by Wnt/β-catenin Signaling Pathway

目的研究淫羊藿苷通过Wnt/β-catenin信号通路抑制卵巢癌细胞CAOV3的增殖。方法通过四甲基偶氮唑盐(MTT)实验选择适当的淫羊藿苷浓度并探讨其对卵巢癌细胞CAOV3生长、增殖的影响,倒置显微镜观察细胞形态学变化,采用荧光实时定量PCR(RT-PCR)法检测β-catenin及Wnt信号通路靶基因c-myc和cyclin D1 mRNA的表达水平,利用Western blot法检测β-catenin、c-myc和cyclin D1的蛋白表达水平。结果 MTT结果显示,淫羊藿苷能显著抑制CAOV3的生长增殖;倒置显微镜下发现淫羊藿苷能使CAOV3细胞形态发生明显变化; RT-PCR检测结果表明淫羊藿苷可降低β-catenin的mRNA的表达以及抑制Wnt信号通路靶基因c-myc和cyclin D1 mRNA的表达; Western blot法检测结果表明淫羊藿苷可下调β-catenin、c-myc和cyclin D1的蛋白表达。结论淫羊藿苷可以抑制人卵巢癌细胞CAOV3增殖,其机制可能是通过抑制Wnt/β-catenin信号通路来实现的。

Objective To study the inhibitory effect of icariin on the growth of ovarian cancer cell line CAOV3 through Wnt/β-catenin signaling pathway.Methods The concentration of icariin and the effect of icariin on the growth and proliferation of ovarian cancer cell line CAOV3 were detected by MTT assay.The morphological changes were observed by inverted microscope.The RT-PCR method was used to detect the mRNA levels ofβ-catenin,c-Myc and cyclinD1 in Wnt signaling pathway.The protein expression levels ofβ-catenin,c-Myc and cyclinD1 were detected by Western blot.Results MTT assay showed that icariin significantly inhibited the proliferation of CAOV3 cells,and the morphological changes of CAOV3 cells were observed under inverted microscope.The results of RT-PCR showed that icariin could decrease the expression ofβ-catenin,c-Myc and cyclinD1 mRNA in Wnt signaling pathway.Western blot analysis showed that icariin could down-regulate the protein expression ofβ-catenin,c-Myc and cyclinD1.Conclusion Icariin can inhibit the proliferation of human ovarian cancer cell line CAOV3,and its mechanism may be achieved by inhibiting Wnt/β-catenin signaling pathway.