乳腺癌肿瘤组织和血浆中miR-21、miR-210的含量及临床意义

Expression and Clinical Significance of MiR-21/MiR-210 in Tissues and Plasma with Breast Cancer

目的探讨miR-21、miR-210在乳腺癌患者肿瘤组织及血浆中的含量,并进一步分析其在乳腺癌中的诊断及预后评价中的价值。方法收集31例乳腺癌患者肿瘤组织及36例癌旁组织,25例乳腺癌患者及21例健康者血浆。采用实时荧光定量PCR技术(real time fluorescence quantitative PCR,RTFQ PCR)检测miR-21、miR-210在乳腺癌患者肿瘤组织及血浆中的含量,应用受试者工作特征(receive operating characteristic,ROC)曲线分析其在乳腺癌中的诊断价值,通过Kaplan-Meier Plotter网站分析其与患者预后的关系。结果与癌旁正常组织比较,miR-21在乳腺癌肿瘤组织中的含量差异有统计学意义(P <0. 05),miR-210含量差异无统计学意义(P> 0. 05);与健康者血浆比较,miR-21、miR-210在乳腺癌患者血浆中的含量差异无统计学意义(P> 0. 05); miR-21在组织中ROC下面积为0. 695; miR-21高含量组患者生存时间明显低于miR-21低含量组(P <0. 05)。结论 miR-21在乳腺癌肿瘤组织中上调,可作为乳腺癌诊断与预后评价指标。

Objective To investigate the expression of miR-21 and miR-210 in tissues and plasma with breast cancer,further to analyze their value in the diagnosis and prognosis of breast cancer.Methods The expression of miR-21 and miR-210 in 31 tumor tissues and 36 normal tissues,25 tumor plasma samples and 21 healthy controls were detected by RTFQ PCR.We analyzed the diagnostic value of miRNA by receive operating characteristic(ROC)curve and the prognosis of patients by Kaplan-Meier Plotter website.Results The levels of miR-21 in tumor tissues were higher than that in normal tissues(P<0.05),whereas the levels of miR-210 were not(P>0.05).There were no significant differences between the tumor plasma samples and healthy controls(P>0.05).The area of miR-21 in tissues under the ROC curve was 0.695.Kaplan-Meier Plotter reveled that higher miR-21 level was correlated with a shorter survival time(P<0.05).Conclusion miR-21 in breast cancer tissues is up-regulated,and it may be served as a novel marker of diagnosis and prognosis in the breast cancer.