筒鞘蛇菰乙酸乙酯部位的化学成分研究

Study on the Chemical Constituents in the Ethyl Acetate Extract of Balanophora involucrate

目的:研究筒鞘蛇菰乙酸乙酯部位的化学成分,为进一步丰富该属植物的化学成分及筒鞘蛇菰的开发利用提供参考。方法:以75%乙醇提取筒鞘蛇菰全株得到醇提物,依次用石油醚、二氯甲烷、乙酸乙酯对提取物进行萃取得到相应部位萃取物。通过硅胶柱、凝胶柱、半制备柱对乙酸乙酯部位萃取物中化学成分进行分离纯化,然后结合波谱(质谱、氢谱、碳谱)数据和文献报道对分离得到的化学成分进行结构鉴定。结果:从筒鞘蛇菰乙酸乙酯部位共分离得到了13个化合物,分别鉴定为pyracanthoside(1)、5,7,3′,5′-四羟基二氢黄酮(2)、柚皮素(3)、高圣草酚(4)、橙皮素(5)、樱花亭(6)、圣草酚(7)、金鱼草素6-O-β-D-吡喃葡萄糖苷(8)、青霉酸(9)、二氢青霉酸(10)、2-甲基-3-呋喃甲酸(11)、5-hydroxymaltol(12)和5,7-二羟基色原酮(13),多为二氢黄酮类化合物。化合物2～13为首次从蛇菰属植物中分离得到。结论:本研究丰富了蛇菰属植物的化学成分,并为筒鞘蛇菰的质量评价奠定了一定基础。

OBJECTIVE:To study the chemical constituents in the ethyl acetate extract of Balanophora involucrate,and to provide reference for further enriching chemical constituent of the plant and the development and utilization of B.involucrate.METHODS:The whole plant of B.involucrate was extracted with 75% ethyl alcohol.The extraction was carried out by petroleum ether,dichloromethane,ethyl acetate and n-butyl alcohol in turn.The chemical compounds from ethyl acetate extract part were isolated and purified by silica gel column,gel column and semi-preparative HPLC.The structures were identified on the basis of spectral spectrum(mass spectrum,hydrogen spectrum and carbon spectrum)data and literature reports.RESULTS:Thirteen compounds were isolated from ethyl acetate extract part of B.involucrate,identified respectively as pyracanthoside(1),5,7,3′,5′-tetrahydroxyflavanone(2),naringenin(3),homoeriodictyol(4),hesperetin(5),sakuranetin(6),eriodyctiol(7),aureusidin-6-O-β-D-glucopyranoside(8),penicillic acid(9),dihydropenicillic acid(10),2-methyl-3-foroic acid(11),5-hydroxymaltol(12)and 5,7-dyhydroxy chromone(13).Most of them were dihydroflavones.Compounds 2 to 13 are isolated from Balanophora genus for the first time.CONCLUSIONS:The study enriched the chemical constituents of the Balanophora genus and lays foundation for quality evaluation of B.involucrate.