摘要
The objective of this study was to examine the eff ects of temperature and light intensity on growth of female gametophytes of Eisenia bicyclis and Ecklonia cava and responses of these female gametophytes to Fe addition and daylength. Female gametophytes of each species were cultured at four temperatures (10, 15, 20, and 25°C) and under a combination of four light intensities (10, 20, 40, and 80 μmol photons/(m^2 ·s)) with two temperatures (15 and 20°C for E i . bicyclis;20 and 25°C for E c . cava ) to clarify their optimal growth conditions. Growth and maturation of female gametophytes of these two species under a combination of five Fe-EDTA concentrations (0, 1, 2, 4, and 8 μmol/L) and three daylengths (10, 12, and 14 h) were also examined. The growth of E i . bicyclis gametophyte was maximal at approximately 15–20°C, 20 μmol photons/(m^2 ·s), Fe-EDTA concentration of 8 μmol/L and daylengths of 12–14 h. While E c . c ava gametophytes showed optimal growth at approximately 20–25°C, 20 μmol photons/(m^2 ·s), Fe- EDTA concentration of 8 μmol/L and daylength of 14 h. Maturation of gametophytes was enhanced at Fe-EDTA concentration of 4 μmol/L for E i . bicyclis and at 2 μmol/L for E c . c ava . In conclusion, optimal growth temperatures and Fe-EDTA concentrations for maturation of E i . bicyclis and E c . c ava gametophytes were diff erent. Higher optimal growth temperature for E c . c ava gametophytes may contribute to its wider geographical distribution compared to E i . bicyclis which has restricted habitats in Korea. This suggests that addition of Fe ion could be used to recover beds of these two species in barren grounds of Korea.
The objective of this study was to examine the ef fects of temperature and light intensity on growth of female gametophytes of Eisenia bicyclis and Ecklonia cava and responses of these female gametophytes to Fe addition and daylength. Female gametophytes of each species were cultured at four temperatures(10, 15, 20, and 25°C) and under a combination of four light intensities(10, 20, 40, and80 μmol photons/(m 2 ·s)) with two temperatures(15 and 20°C for E i. bicyclis; 20 and 25°C for E c. cava) to clarify their optimal growth conditions. Growth and maturation of female gametophytes of these two species under a combination of five Fe-EDTA concentrations(0, 1, 2, 4, and 8 μmol/L) and three daylengths(10,12, and 14 h) were also examined. The growth of E i. bicyclis gametophyte was maximal at approximately15–20°C, 20 μmol photons/(m 2 ·s), Fe-EDTA concentration of 8 μmol/L and daylengths of 12–14 h. While E c. c ava gametophytes showed optimal growth at approximately 20–25°C, 20 μmol photons/(m 2 ·s), FeEDTA concentration of 8 μmol/L and daylength of 14 h. Maturation of gametophytes was enhanced at Fe-EDTA concentration of 4 μmol/L for E i. bicyclis and at 2 μmol/L for E c. c ava. In conclusion, optimal growth temperatures and Fe-EDTA concentrations for maturation of E i. bicyclis and E c. c ava gametophytes were dif ferent. Higher optimal growth temperature for E c. c ava gametophytes may contribute to its wider geographical distribution compared to E i. bicyclis which has restricted habitats in Korea. This suggests that addition of Fe ion could be used to recover beds of these two species in barren grounds of Korea.
