摘要
There exist severe metal pollutions along the Jiulongjiang estuary in South China. In order to unravel the biological eff ects caused by metal pollutions, proteomic responses were investigated by two-dimensional electrophoresis-based proteomics in oysters Crassostrea sikamea from metal pollution sites, Jinshan (JS) and Baijiao (BJ), and a relatively clean site, Jiuzhen (JZ), along the Jiulongjiang estuary. Results indicated that metal pollutions mainly induced cellular injuries, oxidative and immune stresses, and disturbed ion homeostasis in oysters C . sikamea from both JS and BJ sites via diff erential pathways. Furthermore, metal pollution enhanced transcriptional initiation in oysters from JS site. In addition, the Cu and Fe pollution might be indicated by the 78 kDa glucose regulated protein and ferritin GF1 in oysters C . sikamea , respectively. The study confirms that proteomics is a promising approach to characterize the underlying mechanisms of responses to metal pollution in oysters.
There exist severe metal pollutions along the Jiulongjiang estuary in South China. In order to unravel the biological ef fects caused by metal pollutions, proteomic responses were investigated by two-dimensional electrophoresis-based proteomics in oysters Crassostrea sikamea from metal pollution sites, Jinshan(JS) and Baijiao(BJ), and a relatively clean site, Jiuzhen(JZ), along the Jiulongjiang estuary.Results indicated that metal pollutions mainly induced cellular injuries, oxidative and immune stresses,and disturbed ion homeostasis in oysters C. sikamea from both JS and BJ sites via dif ferential pathways.Furthermore, metal pollution enhanced transcriptional initiation in oysters from JS site. In addition, the Cu and Fe pollution might be indicated by the 78 kDa glucose regulated protein and ferritin GF1 in oysters C. sikamea, respectively. The study confirms that proteomics is a promising approach to characterize the underlying mechanisms of responses to metal pollution in oysters.
基金
Supported by the National Natural Science Foundation of China(Nos.41676114,21237004,41506138)
the Qingdao National Laboratory for Marine Science and Technology(No.QNLM201701)
the “Youth Innovation Promotion Association CAS”(No.2015169)
