以‘巨玫瑰’葡萄为母本的胚挽救育种研究

Study on embryo rescue breeding with 'Jumeigui' grape as female parent

以‘巨玫瑰’品种为母本,‘玫瑰香’和‘黄玫瑰’品种为父本进行胚挽救育种,探究各组合的最佳取样时间和胚发育情况。结果表明,以‘黄玫瑰’作父本的胚发育率和萌发率分别为43.74%和18.75%,相较于以‘玫瑰香’作父本的胚发育和萌发情况具有显著优势,两组合杂交胚珠的最佳取样时期均为授粉后63 d,且胚发育培养80 d进行离体胚培养萌发效果较好。

In order to explore the optimum hybrid parents, sampling time and development days for embryo rescue breeding with 'Jumeigui' varieties as female parent and 'Muscat Hamburg' and 'Huangmeigui' as male parents. The results showed that the embryo development rate(43.74%) and germination rate(18.75%) of 'Huangmeigui' as male parent were significantly superior to that of 'Muscat Hamburg' as male parent. The optimum sampling period of hybrid ovules was 63 days after pollination, and the corresponding embryo development rates were 14.38% and 43.74% respectively. The embryo development culture for 80 days had better germinati on effect in vitro.