盐酸芬戈莫德对结肠癌小鼠肿瘤免疫相关因子的影响

Effects of fingolimod hydrochloride on TSGF in colon cancer mice

目的通过盐酸芬戈莫德对CT26荷瘤小鼠肿瘤免疫相关因子功能的影响,探讨盐酸芬戈莫德的抗肿瘤作用机制。方法实验小鼠分为阴性对照组,盐酸芬戈莫德高、中、低剂量治疗组和荷瘤模型组,给药14d,分别检测以下指标:肿瘤生长抑制率、胸腺指数、脾脏指数、小鼠不同组织T细胞干扰素γ(IFN-γ)的分泌、效应T细胞(CD8^+CD3^+T细胞)的比例、不同组织调节性T细胞(CD4^+CD25^+Tregs)白细胞介素10(IL-10)的分泌。结果盐酸芬戈莫德明显升高CD8^+CD3^+T细胞在肿瘤局部的比例(P <0.05);盐酸芬戈莫德显著提高外周血和淋巴结中T细胞分泌IFN-γ的能力(P <0.05);盐酸芬戈莫德有抑制CD4^+CD25^+Tregs分泌IL-10的作用(P <0.05)。结论盐酸芬戈莫德可能通过影响Tregs的免疫抑制功能发挥抗肿瘤的作用。

Objective To investigate the antitumor mechanism of fingolimod hydrochloride through analysis of fingolimod hydrochloride on TSGF in colon cancer mice. Methods The mice were divided into negative control group,fentanyl hydrochloride high,medium and low dose treatment group and tumor-bearing model group,and were given for 14 days.The tumor growth inhibition rate,thymus index,spleen index,the secretion of IFN-γ,the proportion of effector T cells(CD8+CD3+T),and the secretion of interleukin 10(IL-10)by regulatory T cells(CD4+CD25+Tregs) were detected. Results Fingolimod hydrochloride was significantly increased the proportion of CD8+CD3+T cells in local tumor(P<0.05).Fingolimod hydrochloride increased the ability of T cells in peripheral blood and lymph nodes to secrete IFN-γ significantly(P<0.05).Fingolimod hydrochloride inhibited the secretion of IL-10 by CD4+CD25+Tregs(P<0.05). Conclusion Fingolimod hydrochloride may play an anti-tumor role by affecting the immunosuppressive function of Tregs.