延龄草总皂苷AB-8大孔树脂纯化工艺研究

Study on Purification of Total Saponins in Trillium tschonoskii with AB-8 Mmacroporous Resin

目的研究AB-8大孔树脂纯化延龄草总皂苷的工艺。方法以总皂苷和主要皂苷成分{重楼皂苷VII、VI和偏诺皂苷元-3-O-α-L-鼠李糖基(1→4)-[O-α-L-鼠李糖基(1→2)]-O-β-D-葡萄糖苷(PRRG)}的含量为指标,考察延龄草提取物大孔树脂分离的参数,初步确定延龄草总皂苷的制备工艺。结果 AB-8大孔树脂制备延龄草总皂苷的工艺参数为:上样浓度为生药0. 05g/m L,上样量为6倍柱床体积(BV),洗脱流速0. 5mL/min,用蒸馏水(5BV)、20%乙醇(3BV)依次洗脱除去杂质,70%乙醇洗脱(7BV)制备总皂苷,总皂苷的转移率可达87. 30%。结论 AB-8大孔树脂能有效富集和纯化延龄草总皂苷,所确定的工艺条件为该药物的开发利用提供了依据。

Objective To investigate the purification methods of total saponins in Trillium tschonoskii( TSTT) with AB-8 macroporous resin. Methods The dynamic adsorption and desorption experiments have been carried out on a AB-8 resin packed column to optimize the separation process of TSTT from the crude extracts,using the contents of total saponin and the three main compounds( paris saponin VI,VII and pennogenin-3-O-α-L-rhamnopyranosyl( 1→4)-[O-α-L-rhamnopyranosyl( 1→2)〗-O-β-D-glucopyranoside( PRRG)) as indicators. Results The optimum parameters for purification of TSTT by AB-8 resin were as follows:( 1) The concentration of crude drug in a sample solution was 0. 05 g/m L with a processing volume of 6 bed volume( BV),and the flow rate was 0. 5 m L/min;( 2) For desorption,the column was eluented with water( 5 BV),20% ethanol-water( 3 BV) and 70% ethanol-water( 6 BV) successively,and the total saponin were enriched in the 70%ethanol elution with high transfer rate( 87. 30%). Conclusion The preparative separation of TSTT can be easily and efficiently achieved by using AB-8 macroporous resin,the method developed will provide a potential approach for its wide pharmaceutical use.