摘要
ISSR分子标记是为了克服SSR引物设计费时费力的问题,在此发展起来的一种分子标记。其操作简单、高效,试验成本低,且多态性高、重复性好、引物设计简单。本文对几种常用分子标记的优缺点进行简单比较,重点介绍了目前广泛应用于果蔬资源研究中的ISSR分子标记的原理和特点,对ISSR分子标记技术在果蔬资源的遗传多样性和遗传结构、种质资源鉴定以及遗传图谱等方面的研究进展及应用进行综述,旨为果蔬资源的进一步研究利用提供参考依据。
Inter -simple sequence repeat (ISSR) molecular marker is one of molecular markers which is developed to overcome the time - consuming and laborious problem of SSR primer design.lt has simple operation, high efficiency, low experimental cost, high polymorphism, good repeatability and simple primer design.The advantages and disadvantages of several common molecular markers were compared in the article, mainly introduced the principle and characteristics of ISSR molecular marker widely used in fruit and vegetable resources at present.The research progress and application of ISSR molecular marker in the genetic diversity, genetic structure, germplasm resources identification and genetic map of fruit and vegetable resources were reviewed.lt aims to provide a reference for further research and utilization of fruit and vegetable resources.
作者
张珺
孔维鹏
刘丽平
王兴腾
张艺粒
李霞
张小艾
ZHANG Jun;KONG Wei-peng;LIU Li-ping;WANG Xing-teng;ZHANG Yi-li;LI Xia;ZHANG Xiao-ai(College of Horticulture,Sichuan Agricultural University,Chengdu Sichuan 611130)
出处
《现代农业科技》
2019年第4期45-47,共3页
Modern Agricultural Science and Technology
基金
国家自然科学基金(NSFC 31372363)
关键词
ISSR
分子标记
果蔬
遗传多样性
亲缘关系
基因图谱
ISSR
molecular marker
fruit and vegetable
genetic diversity
genetic relationship
gene map
