响应面法优化分子蒸馏提纯烟梗浸膏的工艺

Application of response surface method to optimize purification of tobacco stem extract by molecular distillation

为了提高烟梗浸膏的利用价值,以超临界流体萃取法制备的烟梗浸膏为研究对象,采用响应面法对其分子蒸馏法分离提纯的工艺参数进行了优化,并对所得的精油成分进行了气相色谱质谱联用(GC/MS)检测。结果表明:①在蒸馏温度140℃,刮膜转速246 r/min,冷凝口温度39℃的条件下,分子蒸馏的效果较好,精油得率为10.49%±0.05%。②与烟梗浸膏相比,所得精油中有24种成分的质量分数得到大幅提高,其中桉叶油醇的富集倍数最高(112倍)。③所得精油应用于再造烟叶加香,能够有效提高感官品质。该方法适用于提纯烟梗浸膏,且所得精油可以增强产品的烟香。

To improve the utilization value of tobacco stem extract,molecular distillation was used to separate and purify tobacco stem extract prepared by supercritical fluid extraction.The parameters of molecular distillation were optimized by the response surface method,and the components in the extracted essential oil were determined by GC/MS.The results showed that:1)The highest yield of the essential oil was 10.49%±0.05%,the optimized processing parameters were distillation temperature 140℃,film scraping speed 246 r/min,and condensing temperature 39℃.2)Comparing with tobacco stem extract,the contents(mass fraction)of 24 components in the obtained essential oil increased greatly,and the enrichment of eucalyptol was the highest and reached 112 times.3)The obtained essential oil was used as a flavor for reconstituted tobacco,and the sensory evaluation results indicated positive sensory quality.This method is suitable for purifying tobacco stem extracts,and the obtained essential oil enhances the tobacco-like aroma of reconstituted tobacco products.