沙利霉素对舌鳞癌细胞体外增殖、侵袭和迁移能力的影响

Effects of salinomycin on proliferative, migratory and invasive property of tongue squamous cell carcinoma cells in vitro

目的:探讨沙利霉素对口腔舌鳞癌细胞增殖、侵袭和迁移能力的影响,并初步分析其影响机制。方法:CCK8法检测沙利霉素和顺铂分别作用于CAL-27细胞和EA.hy926细胞后,对细胞增殖的影响;Transwell小室法检测沙利霉素对CAL-27细胞侵袭和迁移能力的作用;Western免疫印迹检测沙利霉素对CAL-27细胞中E钙黏蛋白(E-cadherin)、波形蛋白(vimentin)和β连环蛋白(β-catenin)表达的影响。采用SPSS20.0软件包对数据进行统计学分析。结果:沙利霉素抑制CAL-27细胞的增殖,呈时间、剂量依赖性,且其增殖抑制作用强于顺铂(P<0.05);经过沙利霉素(4μmol/L)处理过的CAL-27细胞,其侵袭和迁移能力均显著低于空白对照组(P<0.05);沙利霉素上调CAL-27细胞中E-cadherin蛋白的表达水平,并且下调vimentin和β-catenin蛋白的表达水平。结论 :沙利霉素能够明显抑制CAL-27细胞的增殖能力,显著减弱CAL-27细胞的侵袭和迁移能力,其机制可能与抑制癌细胞发生上皮-间充质转化(epithelial-mesenchymal transitions,EMT)有关。

PURPOSE: To investigate the effects of salinomycin on proliferative, migratory and invasive properties of tongue squamous cell carcinoma cells, and to explore its possible mechanism. METHODS: CCK8 assay was used to detect the effect of salicamycin and cisplatin on proliferative abilities of CAL-27 cells and EA.hy926 cells. The invasive and migratory ability was detected by Transwell assay. The protein expressions of E-cadherin, vimentin and β-catenin was evaluated by Western blot. SPSS20.0 software package was used to analyze the data. RESULTS: Salicamycin can effectively inhibit proliferation of CAL-27 cells, and the inhibitive ability of salicamycin on the proliferation was stronger than that of cisplatin. CAL-27 cells were treated by salinomycin(4 μmol/L) before invasive and migratory abilities were examined. Compared with control group, the number of invasive and migratory cells in the salinomycin-treated group was significantly decreased(P<0.05). Western blot analysis showed that the protein expression of vimentin and β-catenin was significantly down-regulated. The expression of E-cadherin was significantly increased with the increase of salicamycin concentration. CONCLUSIONS: The proliferative, invasive and migratory ability of CAL-27 cells can be inhibited by salinomycin, which may be related to the inhibition of epithelial-mesenchymal transitions.