光动力疗法对种植体周炎症因子含量的影响

Effect of photodynamic therapy on interleukin in gingival crevicular fluid from patients receiving oral implantation

目的 :通过检测光动力治疗(photodynamic therapy,PDT)前、后患者龈沟液IL-1β、TNF-α、IL-6和IL-17的含量变化,评价Denfotex光动力治疗系统控制炎症的临床效果。方法:对20例磨牙区单牙种植患者行PDT治疗。选用635 nm,100 mW二极管激光,1 mL 12.7 mg/L甲苯胺蓝(TBO),近、远、颊、舌腭4个位点各照射90 s。应用双抗体夹心酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测治疗前、治疗后2周、3个月龈沟液中IL-1β、TNF-α、IL-6和IL-17的含量,光动力疗法分别于基线、2周采样后重复应用。采用SPSS 23.0软件包对数据进行统计学分析。结果:治疗后2周,龈沟液IL-1β、TNF-α含量与治疗前基线相比均有所降低,但无显著差异;治疗后3个月,4种检测因子较治疗后2周及基线均有显著降低(P<0.05)。结论:Denfotex光动力治疗能显著降低种植患者龈沟液中炎症因子含量,可作为有效预防种植体周围炎的一种治疗方法。

PURPOSE: Photodynamic therapy(PDT) has been applied in treatment of peri-implantitis for its antimicrobial effects. The aim of the study was to evaluate the effects of a Denfotex PAD Light System’s photodynamic therapy on prevention of peri-implantitis. METHODS: Twenty patients with implants in molar area were treated with photoactivated disinfection right after completion of suprastructures. The irradiation time was 30 seconds for mesial, distal, buccal and lingual/palatal sites respectively at a power output of 100 mW. Gingival crevicular fluid(GCF) samples from these patients were obtained before photodynamic treatment, 2 weeks and 3 months after treatment. GCF was collected using paper points, and enzyme-linked immunosorbent assay(ELISA) was performed to determine the cytokine(IL-1β,ΤΝF-α, IL-6 and ΙL-17) levels. The data were analyzed with SPSS 23.0 software package. RESULTS: ELISA showed IL-1β andΤΝF-α levels of all patients were decreased 2 weeks and 3 months after treatment. Reduction of IL-1β,ΤΝF-α, IL-6 and IL-17 from 2 weeks to 3 months and from baseline to 3 months all showed a significant difference(P <0.05).CONCLUSIONS: Denfotex PADTMlight system is effective in reducing peri-implant inflammatory mediators and can work as an effective treatment to prevent peri-implantitis.