Bio-Plex悬液芯片技术在婴幼儿喘息综合征诊治中的应用

Application of Bio-Plex suspension array system in diagnosis and treatment of childhood wheezing syndrome

目的探讨Bio-Plex悬液芯片技术在婴幼儿喘息综合征诊治中的应用。方法选择婴幼儿喘息综合征患儿42例,依据相应标准进一步分为特应征组22例和非特应征组20例,对照组20例为健康体检者。采用Bio-Plex悬液芯片技术检测各个血清样本中白细胞介素(IL)-4、IL-6、IL-10及IL-17的含量。结果与对照组相比,婴幼儿喘息综合征组患儿血清中IL-4[(15.9±4.8)pg/mL]、IL-6[(164.5±34.3)pg/mL]、IL-10[(43.7±4.5)pg/mL]、IL-17[(24.7±3.0)pg/mL]明显升高,差异均有统计学意义(P<0.05)。与非特应征组相比较,特应征组患儿血清中IL-4[(18.3±3.9)pg/mL]、IL-6[(190.7±14.5)pg/mL]、IL-10[(48.0±2.1)pg/mL]、IL-17[(26.8±1.9)pg/mL]明显升高,差异均有统计学意义(P<0.05)。结论应用Bio-Plex悬液芯片技术进行检测,发现婴幼儿喘息综合征患儿有多种免疫失衡,存在Th2、Th17、Treg相关细胞因子表达活跃。

Objective To investigate the application of Bio-Plex suspension array system in diagnosis and treatment of wheezing syndrome in children under 5 years old. Methods Forty-two cases of childhood wheezing syndrome were divided into two groups according to the corresponding criteria: wheezing group with atopic body(n=22) and wheezing group without atopic body(n=20). Twenty healthy children were used as the control group. Bio-Plex suspension array system were applied in detecting the levels of IL-4, IL-6, IL-10 and IL-17. Results There was no significant difference among the three groups in ages or genders. Compared with control group, the levels of IL-4(15.9±4.8), IL-6(164.5±34.3), IL-10(43.7±4.5) and IL-17(24.7±3.0) in peripheral blood of the patients with childhood wheezing syndrome were all significantly higher than those of control group(P<0.05). Compared with wheezing group without atopic body, the levels of IL-4(18.3±3.9), IL-6(190.7±14.5), IL-10(48.0±2.1) and IL-17(26.8±1.9) of the patients in wheezing group with atopic body were all significantly higher than those of wheezing group without atopic body(P<0.05). Conclusion There are many immune imbalances and abnormal expression of related cytokines with Th2 cells, Th17 cells and Treg cells in childhood wheezing syndrome according to the application of Bio-Plex suspension array system.