“中脘”“足三里”不同刺激参数组合对正常大鼠胃内压的影响

Influence of electroacupuncture of “Zhongwan” (CV12) and “Zusanli” (ST36) in different combinations of stimulating strength and sequence on intragastric pressure in normal rats

目的:观察"中脘""足三里"不同刺激参数组合对正常大鼠胃内压的影响,探索"中脘""足三里"的配伍运用规律。方法:按照进针顺序("中脘"先针/"足三里"先针)和电针刺激强度组合("中脘"1mA和"足三里"1mA、"中脘"1mA和"足三里"2mA、"中脘"1mA和"足三里"4mA)将SD大鼠随机分为6组,每组15只。在动物胃窦放置球囊,连接压力感受器和多导电生理记录系统并记录胃内压,应用2×3×4析因分析方法分析0～30s、30～60s、60～90s、90～120s4个时间段各组电针干预对胃内压的变化倍率值影响,观察电针"中脘""足三里"不同刺激参数组合在不同时间段对大鼠胃内压的影响。结果:在0～30s时间段内,"中脘"对胃内压的效应以抑制为主(P<0.05),"足三里"穴显示出兴奋的效应趋势(P>0.05)。与0～30s的效应相比,各组电针在30～120s的效应量降低(P<0.01)。在"中脘"1mA和"足三里"1mA刺激下,进针顺序可影响0～30s的效应,对30～120s的效应没有影响;在"中脘"1mA和"足三里"2mA刺激下,进针顺序可影响0～30s和90～120s的效应;在"中脘"1mA和"足三里"4mA刺激下,进针顺序可影响0～120s的效应;"中脘"先针的情况下,"足三里"刺激强度加大到4mA可明显拮抗"中脘"的效应。结论:"中脘"和"足三里"配伍应用可产生拮抗效应,这一效应受进针顺序、电针刺激强度和针刺时间的影响。

Objective To observe the effect of electroacupuncture(EA)of"Zhongwan"(CV12)and"Zusanli"(ST36)in different combinations of stimulating parameters on intragastric pressure(IGP)in normal rats so as to explore their best combinations for promoting gastrointestinal mobility.Methods A total of 90 male SD rats were randomly divided into 6 EA groups:CV12-1 mA+ST36-1 mA,CV12-1 mA+ST36-2 mA,CV12-1 mA+ST36-4 mA,ST36-1 mA+CV12-1 mA,ST36-2 mA+CV12-1 mA,and ST36-4 mA+CV12-1 mA which the first acupoint was stimulated first,followed by the second in each group(n=15 rats/group).Before(1 min),and 0-30 s,30-60 s,60-90 s,and 90-120 sduring EA stimulation of the left ST36 or CV12 first or later,the IGP was measured via an inserted intragastric balloon,a connected pressure transducer and an amplifier.Changes of the IGP were analyzed using 2×3×4 factorial design.Results 1)During 0-30 s,EA-CV12 showed an obvious inhibitory effect on IGP(P<0.05)while EA-ST36 showed a mild exciting effect(P>0.05).2)Compared with the IGP level of 0-30 s,the IGP levels of 30-120 swere significantly decreased in all the groups(P<0.01).3)In the CV12-1 mA/ST 36-1 mA groups,only the IGP level of 0-30 swas affected by the EA-stimulating order(P<0.05).In the CV12-1 mA/ST36-2 mA groups,both the IGP levels during 0-30 sand 90-120 swere obviously affected by EA-stimulating sequence.In the CV12-1 mA/ST36-4 mA groups,the IGP level during 0-120 swas affected by EA-stimulating order.4)Only in the condition of EA-CV12 stimulating first and EAST36 second and at 4 mA,the reduction effect of IGP of EA-CV12 was antagonized.There are marked interaction effects between the EA strength and acupoint EA stimulating sequence,and between the time course and acupoint EA stimulating sequence(P<0.01),but no significant interaction effects were found between the time course and stimulating strength,and among the EA stimulating strength,time course and acupoint EA stimulating sequence(P>0.05).Conclusion Simultaneous EA stimulation of ST36 and CV12 has an antagonistic effect on IGP in normal rats,which is affected by the stimulating sequence,stimulating strength and time course.