银杏叶提取物对牙周炎正畸大鼠牙周组织中IL-6表达影响的研究

Effect of Ginkgo biloba extract on the expression of IL-6 in periodontal tissues in rats with periodontitis

目的探讨银杏叶提取物对牙周炎正畸大鼠牙周组织的影响。方法选取60只雄性Wistar大鼠,随机选取6只为空白对照组,剩余大鼠牙周炎建模,4周后随机选取6只为炎症对照组,其余大鼠建立牙移动模型,分为炎症加力组和炎症加力给药组。炎症加力给药组以灌胃的方式给予大鼠银杏提取物溶液,炎症加力组给予等量生理盐水,每天1次,分别于加力3、7、14、21d后处死大鼠,观察牙移动距离并行HE染色、免疫组化分析。结果炎症加力组牙周组织破坏程度较炎症加力给药组严重,炎症加力给药组大鼠的牙齿移动距离均小于炎症加力组,牙周组织中白细胞介素-6(IL-6)的表达也较炎症加力组低。结论实验选取的银杏叶提取物可以降低牙周组织IL-6的表达,并且使炎性正畸大鼠在牙周组织破坏减小的情况下得到较好的牙移动。

Objective To investigate the effect of of Ginkgo biloba extract on the expression of IL-6 in periodontal tissues in rats with periodontitis.Methods A total of 60 male Wistar rats were included,6 served as blank group and periodontitis models were established in the rest of the rats.Four weeks later,6 were selected as periodontitis control group and orthodontic models were established in the rest which were then divided into in orthodontic group and orthodontic drug delivery group.Ginkgo biloba extrac was administered in orthodontic drug delivery group.The rats were sacrificed after exerting orthodontic force for 3 days,7 days,14 days and 21 days.The distance of tooth movement was observed and the expression of IL-6 examined.Results The damage degree of periodontal tissue in the orthodontic group was more serious than that of the orthodontic drug delivery group.The distance of the tooth movement of the rats in the drug group was lower than that in the orthodontic group,and the expression of IL-6 in the periodontal tissue was also lower than that in the orthodontic group.Conclusion Ginkgo biloba extract can reduce the expression of IL-6 in periodontal tissues and make the tooth move with less periodontal tissue damage.