输入性疖肿型皮肤蝇蛆病一例及致病瘤蝇C0I基因序列分析

A case of imported furuncular cutaneous myiasis and cytochrome oxidase I gene sequence analysis of the pathogenic Cordylobia anthropophaga

报道1例输入性疖肿型皮肤蝇蛆病,并对致病瘤蝇的线粒体细胞色素C氧化酶亚基I(C0I )基因序列进行分析患者女.33岁.患者于1个月前去非洲加纳和喀麦隆两地.期间未采取任何防蚊措施.且有室外晾晒衣物经历皮肤科检查:左上臂内侧和左侧胸部外侧各见一疖性隆起,直径1 ~2cm,鲜红色.边缘不规则,触之较硬,皮损表面中央有小孔.形态学观察显示皮损处挤出的虫体疑似为蝇蛆;PCR扩增虫体C0I基因片段获得650 bp左右的产物.测序后经BLAST比对分析显示,该产物与喀麦隆2010年分离的嗜人瘤蝇C0I基因FR719158.I亲缘关系最近.序列相似性为99.84%,在系统进化树上聚集在一起:综合患者的临床特征、旅行史和致病瘤蝇的序列分析结果.确认该病例为嗜人瘤蝇导致的输入性疖肿型皮肤蝇蛆病.

To report a case of imported furuncular cutaneous myiasis, and to analyze the sequence of the mitochondrial cytochrome C oxidase subunit I (CO I) gene of the pathogenic Cordylobia anthropophaga. A 33-year-old female patient had a travel history to Ghana and Cameroon in Africa 1 month prior to the presentation. No anti - mosquito measures were taken during her stay, and she hung up the laundries outside to dry for several times. Skin examination showed fuiuncular protuberances with diameters of 1 - 2 cm on the inner side of the left upper arm as well as on the outer side of the left chest, which were bright red and hard on palpation with irregular borders and a small hole on their central surface. Morphological identification revealed that the laiva squeezed from the lesion was suspected as myiasis. After PCR amplification of the CO I gene of the larva, an about 650 - bp PCR product was acquired. Sequencing and BLAST analysis showed that this product was most closely related to the CO I gene (GenBank accession number: FR719158.1 ) of Cordylobia anthropophaga isolated in Cameroon in 2010 with the sequence similarity being 99.84%. and they were grouped together on the phvlogenetic tree. According to the clinical features and travel history of the patient and the sequencing results of the pathoge nic Cordylobia anthropophaga. this case was con firmed as imported furun cular cutaneous myiasis caused by Cordylobia anthropophaga.