miRNA-21在黏膜相关淋巴样组织结外边缘区淋巴瘤中的表达及与CHOP方案疗效的关系

Expression of miRNA-21 in mucosa-associated lymphoid tissue lymphomas and its relationship with CHOP regimen

目的探讨m iRNA-21 ( miR-21 )在黏膜相关淋巴样组织结外边缘区(MALT)淋巴瘤中的表达及其与CHOP方案疗效的关系方法收集2015年1月至2017年10月52例就诊于河北工程大学附属医院的经病理证实的MALT淋巴瘤患者的淋巴结组织和治疗前外周血,同时分别收集10例淋巴结炎患者淋巴结组织和20名健康体检者外周血清,采用实时荧光定量聚合酶链反应(qPCR )检测并对比MALT淋巴瘤患者和淋巴结炎患者淋巴结组织、MALT淋巴瘤患者和健康体检者外周血清中miR-21相对表达量。选取20例MALT淋巴瘤患者给予CHOP方案治疗6个周期后,比较治疗的有效率和无效率,同时检测有效组患者的外周血miR-21相对表达量.结果淋巴结炎患者和MALT淋巴瘤患者淋巴结组织中miR-21相对表达量分别为1.03 ±0.12和4.53±0.73,健康体检者和MAI.T淋巴瘤患者治疗前外周血清中miR-21相对表达量分别为0.83 ± 0.04和3.87±0.21,差异均具有统计学意义(P =0.047,P= 0.044 )。MALT淋巴瘤患者CHOP方案治疗6个周期后,治疗总有效率为70 %( 14/20),CHOP方案治疗有效患者治疗前与治疗后外周血清中miR-21相对表达量分别为3.95 ± 0.08和1.62 ±0.41,差异具有统计学意义(P= 0.035 )。结论miR-21与MALT淋巴瘤的发生、发展具有一定的相关性.血清miR-21相对表达量可能与MALT淋巴瘤CHOP方案化疗效果相关。

Objective To investigate the expression of miRNA (miR-21) in mucosa-associated lymphoid tissue(MALT) lymphomas and its relationship with the effects in CHOP regimen. Methods Lymph gland tissues and preoperative peripheral blood of 52 patients pathologically diagnosed with MALT lymphoma in Department of Hematology of Affiliated Hospital of Hebei University of Engineering from January 2015 to December 2017 were collected;meanwhile, 10 tissues from patients with lymphadenitis and 20 peripheral serum from healthy examination patients were also collected. Quantitative real-time polymerase chain reaction (qPCR) was used to compare the lymph gland tissues in MALT lymphoma patients and lymphadenitis patients, and the expression of miR-21 in peripheral serum of MALT lymphoma and healthy people. The selected 20 cases of MALT lymphoma were given CHOP regimen treatment for 6 cycles, and then the efficiency and inefficiency were compared;at the same time, the expression of miR-21 in peripheral serum of the patients in the effective group was detected. Results The relative expression of miR-21 of lymph node tissues in lymphadenitis patients and MALT lymphoma patients was 1.03±0.12 and 4.53±0.73 respectively, and the relative expression of miR-21 in preoperative peripheral serum of healthy people and MALT lymphoma patients was 0.83±0.04 and 3.87±0.21 respectively, and the differences were statistically significant (P = 0.047. P = 0.044). After 6 cycles of CHOP regimen, the total effect rate of MALT lymphoma was 70 %(14/20). and the relative expression of niiR-21 in preoperative and postoperative peripheral serum for the patients who obtained the effective results after CHOP regimen was 3.95 ±0.08 and 1.62 ±0.41. and the differences were statistically significant (P=0.035). Conclusions The expression of niiR-21 has a certain correlation with the occurrence and development of MALT lymphoma. Furthermore. the serum miR-21 expression may be related to the effect of CHOP regimen chemotherapy in MALT lymphoma.