大肿瘤抑制激酶1和大肿瘤抑制激酶2蛋白在胃癌中的表达及其意义

Expressions of large tumor suppressor kinase 1 and large tumor suppressor kinase 2 in gastric cancer and their significances

目的探讨大肿瘤抑制激酶1 ( LATS1 )和大肿瘤抑制激酶2 ( LATS2 )蛋白在胃癌组织中的表达水平及其与胃癌发生、发展的相关性:方法收集2008年9月至2010年6月包头市肿瘤医院病理科胃癌组织蜡块93例及相应癌旁正常组织,采用免疫组织化学方法分别检测LATS1和LATS2蛋白在胃癌和癌旁正常组织中的表达。采用χ^2检验比较LATS1和LATS2蛋白在胃癌和癌旁正常组织中表达的差异,分析LATS1和LATS2蛋白表达与患者临床病理特征之间的相关性。结果胃癌组织中,LATS1阴性54例(58.1%),弱阳性15例(16.1%),中等阳性16例(17.2%),强阳性8例(8.6%);癌旁正常组织中,LATS1阴性17例(18.3%),弱阳性16例(17.2%),中等阳性31例(33.3%),强阳性29例(31.2%)。LATS1在胃癌组织中的阳性表达率低于癌旁正常组织,差异有统计学意义(χ^2= 37.460,P<0.01 )。胃癌组织中,LATS2阴性28例(30.1%),弱阳性17例(18.3%),中等阳性33例(35.5%),强阳性15例(16.1%);癌旁正常组织中.LATS2阴性5例(5.4%),弱阳性7例(7.5%),中等阳性32例(34.4%),强阳性49例(52.7%)。LATS2在胃癌组织中的阳性表达率低于癌旁正常组织,差异有统计学意义(χ^2 = 38.275,P<0.01 )。LATS1和LATS2蛋白表达与患者年龄、性别及肿瘤淋巴结转移、分化程度、肿瘤直径均无关(均P>0.05);结论LATS1和LATS2蛋白可能参与了胃癌的发生,具有抑制胃癌发生、发展的作用。

Objective To investigate the expressions of large tumor suppressor kinase 1 (LATS1) and large tumor suppressor kinase 2 (LATS2) proteins in gastric cancer tissues, and to explore the correlation between expressions of LATS1 and LATS2 proteins and the occurrence and development of gastric cancer. Methods A total of 93 gastric cancer paraffin tissues and the corresponding adjacent gastric normal mucosa in the Department of Pathology in Baotou Cancer Hospital from September 2008 to June 2010 were collected. The immunohistochemistry was used to detect the expressions of LATS1 and LATS2 proteins in gastric cancer and adjacent normal tissues. The differences of the expressions of LATS1 and LATS2 proteins in gastric cancer and adjacent normal tissues were compared by using χ^2 test. The relationship between the expressions of LATS1 and LATS2 proteins and the clinicopathological features was also analyzed. Results In gastric cancer tissues, LATS1 was negatively expressed in 54 cases (58.1%), weakly positive expressed in 15 cases (16.1%), moderately positive expressed in 16 cases (17.2%), and strongly positive expressed in 8 cases (8.6%);in adjacent normal tissues, LATS1 was negatively expressed in 17 cases (18.3%), weakly positive expressed in 16 cases (17.2%), moderately positive expressed in 31 cases (33.3%), and strongly positive expressed in 29 cases (31.2%). The positive expression rate of LATS1 in gastric cancer tissues was lower than that in adjacent normal tissues, and the difference was statistically significant (χ^2=37.460, P<0.01). In gastric cancer tissues, LATS2 was negatively expressed in 28 cases (30.1%), weakly positive expressed in 17 cases (18.3%), moderately positive expressed in 33 cases (35.5%), strongly positive expressed in 15 cases (16.1%);in adjacent normal tissues, LATS2 was negatively expressed in 5 cases (5.4%), weakly positive expressed in 7 cases (7.5%), moderately positive expressed in 32 cases (34.4%), strongly positive expressed in 49 cases (52.7%). The positive expression rate of LATS2 in gastric cancer tissues was lower than that in adjacent normal tissues, and the difference was statistically significant (χ^2=38.275, P<0.01). The expressions of LATS1 and LATS2 were not related to patients'age, gender, lymph node metastasis, degree of differentiation and tumor diameter (all P>0.05). Conclusion LATS1 and LATS2 proteins may be involved in the occurrence of gastric cancer and have the inhibiting effect on the occurrence and development of gastric cancer.